| 陈立 张英 王雨 李珅 李娜 于炜婷 马小军.增强微胶囊在血液中稳定性的研究[J].现代生物医学进展英文版,2015,15(10):1812-1815. |
| 增强微胶囊在血液中稳定性的研究 |
| Improvement of Stability of Microcapsules in the Blood |
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| DOI： |
| 中文关键词: 微胶囊 稳定性 生物人工肝 |
| 英文关键词: Microcapsules Stability Bioartificial liver |
| 基金项目:国家自然科学基金项目（210762072；81171550; 81101757）；中国科学院战略性先导科技专项（XDA01030303） |
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| 中文摘要: |
| 目的：通过改进成膜工艺，减少血液中HCO3
-对海藻酸钠-ε - 聚赖氨酸（ε -AP）微胶囊稳定性的影响，从而增强ε -AP微胶
囊在血液中的稳定性，使其能够应用于生物人工肝血浆/ 血液灌注。方法：改进成膜工艺：在成膜液中添加10 mmol/L的NaHCO3
并增加ε - 聚赖氨酸反应浓度来制备ε -AP 微胶囊，用膨胀度表征其机械强度，荧光标记的牛血清白蛋白（FITC-BSA）检测其通
透性能，并以微胶囊的膨胀度变化作为衡量指标，考察微胶囊在NaHCO3溶液和血清中的稳定性。结果：改进成膜工艺制备的
ε -AP微胶囊与常规条件制备的微胶囊具有接近的机械强度和通透性；改进成膜工艺能够增强的ε -AP 微胶囊在NaHCO3溶液
和血清中的稳定性；常规条件制备的ε -AP微胶囊在血清中膨胀度增加了约560%，而改进成膜工艺制备的微胶囊膨胀度仅增加
160%，其稳定性显著增强。结论：改进成膜工艺能够增强ε -AP微胶囊在血液中的稳定性。 |
| 英文摘要: |
| Objective:To reduce the impact of HCO3
- on the stability of alginate-ε -poly (L-lysine) microcapsules (ε -AP) and
improve the stability of ε -AP microcapsules in the blood by adjusting preparation condition for potential application of
microcapsule-based bioartificial livers.Methods:ε -AP microcapsules were obtained by adjusting preparation condition. Specifically, 10
mmol/L NaHCO3 was added to the microcapsule-forming solution and the concentration of ε -poly (L-lysine) was increased. The
mechanical stability of microcapsules was measured by swelling degree and the permeability of microcapsules was evaluated by
FITC-labeled bovine serum albumin (BSA). Then, the stability of microcapsules in NaHCO3 solution and in the blood was studied and
measured by the change of swelling degree.Results:Microcapsules were prepared with their mechanical stability and permeability
similar to those obtained under conventional preparation condition. The stability of ε -AP microcapsules in the NaHCO3 solution and in
the blood was improved by adjusting preparation condition. The swelling degree of conventionally prepared microcapsules in the blood
was increased by 560%, while the swelling degree of microcapsules obtained by adjusting preparation condition was only increased by
160%. The stability of microcapsules was significantly improved.Conclusion:The stability of ε -AP microcapsules was greatly
improved by adjusting preparation condition. |
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