| 孙全波 栾晓 郭菲菲 孙向荣 祝海 逄明杰 王萍 谷方 徐珞.Obestatin 对血浆ghrelin和nesfatin-1水平及胃排空的影响[J].现代生物医学进展英文版,2015,15(9):1628-1631. |
| Obestatin 对血浆ghrelin和nesfatin-1水平及胃排空的影响 |
| The Effects of Obestatin on Plasmaacyl Gheelin and Nesfatin-1 Levels,and on Gastric Emptying in Rats |
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| DOI： |
| 中文关键词: 胃排空 Ghrelin Nesfatin-1 Obestatin |
| 英文关键词: Gastric emptying Ghrelin Nesfatin-1 Obestatin |
| 基金项目:国家自然科学基金项目（31071014；81100260；81300281；81470815；81270460）；青岛市科技局项目（13-1-4-170-jch；14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨侧脑室注射obestatin 对大鼠血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1 水平的影响以及对胃排空的调控。方
法：侧脑室注射obestatin，采用酶免疫测定（EIA）法检测血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1 水平以及胃排空率的变
化。结果：侧脑室分别注射0.1、0.3 或1.0 nmol obestatin，大鼠血浆酰基化ghrelin、去酰基化ghrelin 以及nesfatin-1 水平无显著改
变（P>0.05），且酰基化ghrelin 与去酰基化ghrelin 比率无显著改变（P>0.05）；侧脑室注射obestatin，大鼠摄食量无显著改变，但胃
排空率明显增加（P<0.05）；胃排空率明显延迟（P<0.05）。与侧脑室注射1.0 nmol Obestatin 组相比，注射1.0 nmol Obestatin+CRF，
大鼠摄食量无显著改变，胃排空率明显延迟（P<0.05）。各组摄食量及进入十二指肠内食物量无明显差异（P>0.05）。结论：中枢
obestatin 促进大鼠的胃排空，可能与h/rCRF通路有关。 |
| 英文摘要: |
| Objective:To discuss the impact of intracerebroventricular obestatin on plasma acyl ghrelin, des-acyl ghrelin and
nesfatin-1 levels, and the regulation of gastric emptying in rats.Methods:Rats were administrated obestatin through
intracerebroventricular (ICV) injection; Enzyme immunoassay (EIA) method was used to detect the plasma levels of acyl ghrelin,
des-acyl ghrelin, nesfatin-1 and changes in the rate of gastric emptying.Results:After lateral ventricle injection respectively 0.1, 0.3 or
1.0 nmol obestatin, the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 had no significant change (P>0.05), and the ratio of acyl
ghrelin and des-acyl ghrelin had no significant difference (P>0.05) too. Food intake of rats had no obvious difference after lateral
ventricle injection of obestatin, but the gastric emptying rate increased significantl (P<0.05). Gastric emptying rate delayed significantly
(P<0.05). Compared with lateral ventricle injection 1.0 nmol Obestatin group, injection 1.0 nmol Obestatin + CRF, no significant changes
in food intake in rats significantly delayed gastric emptying rate (P<0.05). While the amount of food intake and the food into the
duodenum had no obvious difference (P>0.05).Conclusion:Central obestatin promotes gastric emptying of rats, which may be
associated with h/rCRF pathways. |
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