| 蔡俊玮 李雪锋 黄成虎 徐焱成 曾玉琴.阻断RAS对HSkMCs细胞株细胞凋亡及Mfn2 表达的影响[J].现代生物医学进展英文版,2015,15(8):1449-1451. |
| 阻断RAS对HSkMCs细胞株细胞凋亡及Mfn2 表达的影响 |
| The Effects of Blocking Renin-angiotensin Systemon the Expression of Mfn2mRNA and Apoptosis in HSkMCs |
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| DOI： |
| 中文关键词: 2型糖尿病 肾素-血管紧张素系统（RAS) 胰岛素抵抗 线粒体融合蛋白2（Mfn2） |
| 英文关键词: Type 2 diabetes Renin angiotensin system(RAS) Insulin resistance Mitofusin 2(Mfn2) |
| 基金项目:湖北省教育厅科学技术研究计划重点项目（D20112105）；湖北省十堰市科技局项目（2010st12）；
湖北医药学院2010 博士启动项目（2010QDJ24）；湖北医药学院附属太和医院2010博士启动项目（2010QD15） |
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| 中文摘要: |
| 目的：观察氯沙坦对高糖培养人骨骼肌细胞（Human skeletal muscle cells,HSkMCs）中线粒体融合蛋白2(mitofusin2, Mfn2)的
表达及其对细胞凋亡的影响。方法：1.使用不同浓度的葡萄糖养基（葡萄糖浓度分别为5.55 mmol/L，11.1 mmol/L，22.2 mmol/L）分
别培养HSkMCs 细胞株48 小时，检测各组细胞中血管紧张素Ⅰ型受体(Angiotensin II type I receptor, AT1R)基因、基因Mfn2 的表
达，并用流式细胞术检测细胞凋亡。2. 根据1 中实验结果，选择对Mfn2 影响最大的葡萄糖浓度（此组葡萄糖浓度为22.2
mmol/L）作为后续实验的条件。加入血管紧张素受体Ⅱ拮抗剂（Angiotensin ReceptorBlockers,ARB）氯沙坦（Losartan)，处理人骨骼
肌细胞（HSkMCs）48 h，以未加氯沙坦为对照组，观察其对线粒体融合蛋白2（Mfn2 表达的影响，并行流式细胞术检测细胞凋亡。
结果：氯沙坦干预组HSkMCs细胞中Mfn2 表达上调，细胞凋亡减少。结论：阻断肾素血管紧张素系统(Renin-angiotensin System ，
RAS)能上调HSkMCs细胞株中的Mfn2 表达，并减少细胞凋亡。 |
| 英文摘要: |
| Objective:To observe the effect of losartan on the expression of Mfn2 mRNA and apoptosis in Human skeletal muscle
cells (HSkMCs) cultured by high glucose medium.Methods:HSkMCs were cultivated and treated with glucose of different
concentration (5.55 mmol/L group, 11.1 mmol/Lgroup, 22.2 mmol/Lgroup) for 48 hours. Then the expression of AT1R and Mfn2 was
detected by PCR, apoptosis was detected by flow cytometry.2. Select 22.mmol/Lcgroup which has the most influence on the expression
of Mfn2 from the above experiments as the following experimental conditions. Added losartan ( ARB ) to treat HSkMCs for 48 h, in
compared with the group without losartan. To observe its effect on the expression of Mfn2 and apoptosis via flow cytometry.Results:The
expression of Mfn2 in HSkMCs cultivated added losatan group was up - regulated, while apoptosis was reduced.Conclusion:Blocking
RAS could up-regulate the expression of Mfn2 and reduce cell apoptosis in HSkMCs. |
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