| 秦玺 李永红 杨琦 杨靖清 于雷 杨玉帅 徐莉 饶春明.实时荧光定量PCR 法用于重组SIV-hPEDF注射液病毒颗粒数的检测[J].现代生物医学进展英文版,2015,15(8):1401-1405. |
| 实时荧光定量PCR 法用于重组SIV-hPEDF注射液病毒颗粒数的检测 |
| Quantitation of Virus Particles in Recombinant SIV-hPEGF Injection byReal-Time PCR |
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| DOI： |
| 中文关键词: 重组SIV-hPEDF注射液 实时定量PCR 病毒颗粒数 质量控制 |
| 英文关键词: Recombinant SIV-hPEGF Injection Real-time PCR Virus particles Quality control |
| 基金项目:863 计划项目（2012AA020805）；国家科技重大专项项目（2012ZX09304010） |
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| 中文摘要: |
| 目的：建立重组SIV-hPEDF注射液病毒颗粒数检测实时定量PCR 检测方法。方法：在提取重组SIV-hPEDF注射液供试品基
因组RNA 后，采用实时定量PCR 的方法，检测供试品中的病毒颗粒数。结果：经过3 次实验，所得SIV-hPEDF RNA标准品标准
曲线均线性良好，重组SIV-hPEDF 注射液供试品中病毒颗粒数检测结果无显著性差异。结论：所建立的重组SIV-hPEDF注射液
病毒颗粒数实时荧光定量PCR 检测方法重复性好、灵敏、准确，可用于重组SIV-hPEDF注射液病毒颗粒数的检测。 |
| 英文摘要: |
| Objective:A Real-Time PCR method was developed to quantitatively determine the virus particles in recombinant
SIV-hPEGF injection.Methods:After extracting the genome RNA in recombinant SIV-hPEDF injection, Real-time PCR was used to
quantitatively determine the virus particles in test sample.Results:After 3 parallel experiments, the SIV-hPEDF RNA standard had a
good linear, and there was no significant difference in the detection results of virus particles in recombinant SIV-hPEGF injection.Conclusion:The established method is repeatable, sensitive and accurate, and can be applied in the determination of virus particles in
recombinant SIV-hPEGF injection. |
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