| 李波 肖凤仪 李小洲 王俊耐 杨洁.生长抑素对 Hela 细胞中 Claudin-3 和 Claudin-4 基因表达的调节作用[J].现代生物医学进展英文版,2015,15(7):1249-1252. |
| 生长抑素对 Hela 细胞中 Claudin-3 和 Claudin-4 基因表达的调节作用 |
| The Regulation of Somatostatin on Claudin-3 and Claudin-4 GeneExpression in Hela Cells |
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| DOI： |
| 中文关键词: 生长抑素 Hela 细胞 Claudin-3 Claudin-4 |
| 英文关键词: Somatostatin Hela cells Claudin-3 Claudin-4 |
| 基金项目:国家自 然科学基金项目（ 81201639/H1606） |
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| 中文摘要: |
| 目的： 探讨生长抑素对 Hela 细胞的生长调控作用以及对 claudin-3 和 claudin-4 基因的表达调控。 方法： 通过 Hela 细胞株培
养， 并以浓度为 10-6、 10-8、10-10 和 10-12 M 的生长抑素（ SST）作用 于 Hela 细胞， 未经药物处理的细胞设为对照组。在处理后采用 流式
细胞仪检测 Hela 细胞的凋亡。 并采用 实时荧光定量 PCR 和 Western blot 分别 检测 claudin-3 和 claudin-4 mRNA 和蛋白 质表达
量。 结果： SST 加入 Hela 细胞孵育 12 h 小时后， 10-10 M、 10-8 M 和 10-6 M 浓度的 SST 对 Hela 细胞有显著性的诱导凋亡作用。不同
浓度的 SST 作用 于 Hela 细胞 12 h 后， claudin-3 和 claudin-4 的 mRNA 和蛋白表达量都出现不同水平的增加。 结论： 在 Hela 细胞中
SST 可以促进 claudin-3 和 claudin-4 的基因 表达，从而对宫颈癌的发展和扩散有抑制作用 。 |
| 英文摘要: |
| Objective:To investigate the regulation of somatostatin on Hela cell growth as well as claudin-3 and claudin-4 gene
expression.Methods:Hela cells were treated with 10-6, 10-8, 10-10 and 10-12 M somatostatin (SST), respectively. Untreated cells were used
as control. Flow cytometry was used to detect the apoptosis of Hela cells. Realtime-PCR and Western blot were performed to assay the
effect of SST on mRNA and protein expression of gene claudin-3 and claudin-4.Results:After treatment with SST by differential concentrations for 1 2 h, it was found that 10-10 M, 10-8 M and 10-6 M SST could induce a significant apoptosis in the Hela cells. Under different concentrations of SST, the mRNA and protein expression of claudin-3 and claudin-4 were up-regulated.Conclusion:In Hela cells,
SST could induce claudin-3 and claudin-4 gene expression and inhibit development and diffuse of cervical cancer cells. |
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