麦秀英 熊建锐 王旭磊 李 萍.八氢番茄红素合成酶( PSY)的生物信息学分析[J].现代生物医学进展英文版,2015,15(7):1217-1221. |
八氢番茄红素合成酶( PSY)的生物信息学分析 |
Bioinformatic Analysis of Phytoene Synthase |
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DOI: |
中文关键词: 八氢番茄红素合成酶 生物信息学分析 番茄红素 |
英文关键词: PSY Bioinformatic analysis Lycopene |
基金项目:教育部留学回国人员科研启动基金( 2013S03008) |
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中文摘要: |
目 的: 通过生物信息学方法对八氢番茄红素合成酶基因 (PSY)及氨基酸序列 分析, 并构建三维结构。 方法: 运用 生物信息学
方法对八氢番茄红素合成酶基因及其蛋白质序列 的理化性质、亲 / 疏水性、信号肽、跨膜结构域、糖基化位点, 磷酸化位点, 二级结
构,功能结构域和三级结构进行预测分析。 结果: PSY 基因 含 1 239bp 的开放阅读框,编码氨基酸数为 412,为 碱性不稳定蛋白 ; 八
氢番茄红素合成酶富含 Arg、Leu、 Ala、 Ser、 Val 等氨基酸,为 亲水性蛋白 质; PSY 为 非跨膜蛋白 ,不 含信号肽, 具有多 个磷酸化位
点,alpha 螺旋和无规卷曲是其主要结构元件。 结论: 用 同 源建模的方法构建其三维结构, 得到合理模型 ,为 采用 生物工程提高番茄红
素产量提供理论依据。 |
英文摘要: |
Objective:To analyze the gene and amino acid sequence of eight hydrogen lycopene synthase (PSY) via bioinformatical methods, and build a three-dimensional structure.Methods:This research aimed to predict and analyze the physical and chemical
properties, hydrophilicity and hydrophobicity, signal peptide, transmembrane domains, glycosylation and phosphorylation sites, secondary and tertiary structure of PSY gene and protein sequence by bioinformatic methods.Results:PSY gene is an alkali labile protein,
which contains a long open reading frame of 1 239bp, encodes 412 amino acid; PSY is hydrophilic protein, which is rich in Arg, Leu, Ala,
Ser, Val etc; PSY is non-transmembrane protein, which has no signal peptide and contains several phosphorylation sites, alpha helix and random coil is its primary secondary structural elements.Conclusion:The reasonable three-dimensional structure of PSY was obtained by
using homology modeling software.The research offer a theoretical basis to improve the yield of lycopene in biotechnology. |
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