| 于晓敏 1 宋克娟 2 吕 腾 2 焦今文 2 姚 勤.上皮性卵巢癌患者血清 microRNA-21 的水平及其临床意义研究[J].现代生物医学进展英文版,2015,15(6):1050-1053. |
| 上皮性卵巢癌患者血清 microRNA-21 的水平及其临床意义研究 |
| Expression of MicroRNA-21 in Serum of Patients with Epithelial OvarianCarcinoma and Its Clinical Significances |
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| DOI： |
| 中文关键词: 上皮性卵巢癌 microRNA-21 化疗耐药 Taqman 探针 实时荧光定量 PCR |
| 英文关键词: Epithelial ovarian cancer MicroRNA-21 Drug-resistant Taqman probe Realtime RT-PCR |
| 基金项目:国家自 然科学基金项目（ 81 100697/H1 208）； 山东省博士基金项目（ BS2011 SW003） |
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| 中文摘要: |
| 目的： 检测上皮性卵巢癌患者血清中 microRNA-21 的表达， 并探讨其作为 标记物预测上皮性卵巢癌化疗 耐药患者预后的可
行性。 方法： 采用探针型实时荧光定量逆转录聚合酶链反应检测和比较 20 例上皮性卵巢癌患者和 10 例 正常人卵巢血清标本中
microRNA-21 的表达， 并分析血清 microRNA-21 水平与上皮性卵巢癌患者化疗 耐药及其临床病理特征的相关性。 结果： 正常人、
上皮性卵巢癌化疗敏感和化疗耐药患者血清中 microRNA-21 的相对表达量分别 为 0.573± 0.318、 2.606± 1.057、 26.766± 26.710，
上皮性卵巢癌化疗敏感和化疗 耐药患者血清中 miR-21 的相对表达量均显著高于正常人血清中 miR-21 的相对表达量(P<0.05)，
而上皮性卵巢癌化疗 耐药患者血清中 miR-21 表达显著高于上皮性卵巢癌化疗敏感患者， 差异有统计学意义(P<0.05)。 上皮性卵
巢癌化疗耐药患者血清 miR-21 表达水平与其手术 - 病理分期无及是否发生淋巴结转移均无明显相关性(P>0.05)。 结论： 上皮性卵
巢癌患者血清 microRNA-21 水平显著升高， 可能作为 其化疗耐药的预测参考指标， 但血清 microRNA-21 水平与上皮性卵巢癌化
疗 耐药患者的不良预后并无显著相关性。 |
| 英文摘要: |
| Objective:To detect the expression of microRNA-21 in epithelial ovarian cancer serum samples and investigate the
feasibility of microRNA-21 used as drug-resistant biomarker for the prognostic prediction of drug-resistant ovarian cancer.Methods:The
expressions of microRNA-21 in serum from 20 cases of epithelial ovarian cancer and 10 cases of normal population were detected and
compared by Taqman real-time RT-PCR. The correlations of serum levels of microRNA-21 and drug-resistace and clinicopathological
characteristics of epithelial ovarian cancer were analyzed.Results:The serum levels of microRNA-21 in normal population,drug-sensitive
and drug-resistant epithelial ovarian cancer were respectively 0.573 ± 0.318, 2.606 ± 1 .057, 26.766 ± 26.710. The serum levels of
microRNA-21 in drug-sensitive and drug-resistant epithelial ovarian cancer were both significantly higher than that of normal population
(P<0.05). While the serum levels of microRNA-21 in drug-resistant epithelial ovarian cancer was significantly higher than that of
drug-sensitive epithelial ovarian cancer (P<0.05). The serum levels of microRNA-21 in drug-resistant epithelial ovarian cancer had no
correlation with surgical-pathological stages and lymph nodes metastasis (P>0.05).Conclusion:The serum levels of microRNA-21 in
epithelial ovarian cancer was upregulated, which could be used as a reference index for predicting the chemotherapy drug-resistance. But
the serum level of microRNA-21 had no correlation with the poor prognosis of patients with drug-resistant epithelial ovarian cancer. |
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