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目的：对因自身抗体引起 ABO 血型正反定型结果不一致的疑难血标本进行血型鉴定和分析，探讨其原因和解决办法。方法：先用直接抗人球蛋白试验（ DAT）和间接抗人球蛋白试验（ IAT）确定 ABO 血型正反定型结果不一致的原因是由于自身抗体的存在，然后做吸收放散试验、抗体筛选试验等排除自身抗体的干扰以便血型的正确判定。结果： ABO 血型正反定型结果不一致因自身抗体引起的有 10 例，其中温抗体 7 例，冷抗体 3 例，温抗体同时同种抗体阳性 2 例，冷抗体同时同种抗体阳性 2 例。结论：自身抗体会影响 ABO 血型的正确判定，选用合适的试验进行分析判断以提高血型鉴定的准确性。

英文摘要:

Objective:To analyze the difficult blood samples whose positive and negative stereotypes inconsistent in ABO blood group caused by autoantibody, and to explore the reasons and solutions.Methods:First, the reason of positive and negative stereotypes inconsistent in ABO blood group was found to be the antibodies with DAT and IAT. Then absorption-elution test and antibody screening test was done to eliminate the interference of autoantibodies in order to determine blood type correctly.Results:Antibodies caused positive and negative stereotypes inconsistent in ABO blood group in 1 0 cases: warm antibody type in 7 cases, cold antibody type in 3 cases, warm antibody combine irregular antibody in 2 cases, and cold antibody combine allo-antibody in 2 cases.Conclusion:Autoantibody can affect accurate judgment of ABO blood group, and appropriate tests should be chosen to improve the accuracy of the blood typing.

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