| 杨 蓉 孙 萍 宋芳霞 郝 炯 王 建.卵巢上皮性癌中干扰素介导的跨膜蛋白 1 的表达意义[J].现代生物医学进展英文版,2015,15(5):839-843. |
| 卵巢上皮性癌中干扰素介导的跨膜蛋白 1 的表达意义 |
| Expression and Significance of IFITM1 in Epithelial Ovarian Carcinoma |
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| DOI： |
| 中文关键词: 卵巢肿瘤 IFITM1 基因 免疫组织化学 耐药 |
| 英文关键词: Ovrian neoplasms Interferon-induced transmembrane protein 1 (IFITM1) Immunohistochemistry Drug resistance |
| 基金项目:国家自然科学基金项目 (811 72458) |
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| 中文摘要: |
| 目的： 探讨干扰素介导的跨膜蛋白 1（ Interferon-induced transmembrane protein 1， IFITM1）基因 在卵巢上皮性癌中表达的相
关性及其意义。 方法： 应用 Western blotting 检测正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中 IFITM1 蛋白表
达。 免疫组织化学检测 12 例 正常卵巢、21 例卵巢良性肿瘤、 18 例卵巢交界性肿瘤和 85 例卵巢上皮性癌组织中 IFITM1 的蛋白表
达， 同时分析 IFITM1 表达状况与 临床病理因 素之间 的相关性。 结果： Western blotting 显示卵巢上皮性癌和卵巢交界性肿瘤中
IFITM1 表达水平明显高于正常卵巢组织和卵巢良性肿瘤。免疫组化显示在正常卵巢组织中 IFITM1 阳性表达率为 41.7 %（ 5/12），
在卵巢良性肿瘤组织中 71.4 %（ 15/21），在卵巢交界性肿瘤组织中为 72.2 %（ 1 3/18），在卵巢上皮性癌中为 77.6 %（ 66/85）， IFITM1
蛋白表达强度在正常卵巢、良性卵巢肿瘤、交界性卵巢肿瘤、上皮性卵巢癌间的比较有统计学意义（ P<0.05）。 IFITM1 蛋白表达与
病理类型、肿瘤分化程度、肿瘤 FIGO 分期有关（ P<0.05），与 淋巴结转移、腹水无明显相关性。化疗敏感组和耐药组的 IFITM1 表达
强度间差异有统计学意义（ P<0.05）。 结论： IFITM1 在正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤和卵巢上皮性癌组织中的表达依
次升高， 并与 卵巢癌以铂类为 基础的化疗 耐药性产生有相关性，为 进一步研究 IFITM1 在卵巢癌诊治及化疗中的应用 前景提供依
据。 |
| 英文摘要: |
| Objective:To investigate the role of Interferon-induced transmembrane protein 1 (IFITM1 ) in the carcinogenesis and
evaluate its clinical significance in epithelial ovarian carcinoma.Methods:The expression level of IFITM1 was detected by the Western
blot analysis in different ovaries, and the immunohistochemical method (SP) was used to determine the expression levels of IFITM1
among 1 2 normal ovarian tissue cases, 21 benign ovarian tumor cases, 1 8 borderline ovarian tumor cases and 85 ovarian carcinoma cases.
Then the relationship between the expression level of IFITM1 in ovarian carcinoma and the clinicopathological features was analyzed by
statistics.Results:Western blot analysis showed that the expression levels of IFITM1 differed in different ovarian tissues. The immunohistochemical technique showed that the positive rate of IFITM1 expression was 77.6% (66/85) in ovarian carcinoma, higher than that of
ovarian (41 .7 %, 5/12), benign ovarian tumor (71.4 %, 15/21) and borderline ovarian tumor (72.2 %, 13/1 8) with a dramatical statistic
significance (P<0.05). The expression level ofIFITM1 was correlated with pathology type, tumor grad and FIGO stage in ovrian carcinoma
(P<0.05). The expression intensity of IFITM1 protein in ovarian carcinoma was significantly related to its chemotherapy sensitivity.Conclusion:The expression levels ofIFITM1 gradually increased in normal ovarian tissue, benign ovarian tumor, borderline ovarian tumor
and ovarian carcinoma. Positive expression level of IFITM1 played a vital role in carcinogenesis and progression of ovarian carcinoma
including the drug-resistance to platinum-based chemotherapy. Our research can provide better evidence to the further study of IFITM1
in diagnosis and targeted treatment of ovarian carcinoma. |
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