| 蔡启茵 任广立 张卫云 马恒灏 黄定鹏.乙型肝炎病毒感染 Babl/c 乳鼠动物模型的研究[J].现代生物医学进展英文版,2015,15(5):815-817. |
| 乙型肝炎病毒感染 Babl/c 乳鼠动物模型的研究 |
| Establishment of Babl/c Mouse Model by Infecting Hepatitis B Virus Serum |
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| DOI： |
| 中文关键词: 乙型肝炎 乙型肝炎病毒 Babl/c 乳鼠 |
| 英文关键词: Hepatitis B Hepatitis B virus Babl/c suckling mice |
| 基金项目:广州市科技计划项目 基金项目（ 201 3J4100116） |
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| 中文摘要: |
| 目的： 目前 HBV 感染动物模型各有局限，无法全面研究 HBV。 拟建立人 HBV 血清感染 Babl/c 乳鼠的动物模型， 以便于研
究 HBV 感染与 乳鼠免疫力低下的相关性。 方法： 将 20 只 Babl/c 乳鼠随机分为实验组、对照组、 PBS 组及空白组， 通过高压水动力
尾静脉注射法将人 HBV 血清、正常人血清、 PBS 注入各组乳鼠体内 ，记录接种后乳鼠体温及体质量。 于接种后第 7、 15、30 d 采集
血清标本， 应用 ELISA 检测 HBsAg、HBeAg 的表达情况， 实时荧光定量 PCR 检测 HBV DNA 浓度。 结果： 接种后各组小鼠体温及
体质量均无明显变化。 实验组中共 4 只乳鼠可检测到 HBsAg 为阳性且维持时间 长达 30 d， 但 HBeAg 均为阴 性， HBV DNA 浓度
均未达 500 IU/ml； 余下各组检测 HBsAg、 HBeAg 均 为 阴 性。 结论： 通过高压水动力 尾静脉注射法接种人 HBV 血清， 可成功使
Babl/c 乳鼠感染 HBV。 本实验证实乳鼠免疫力低下， HBV 血清进入体内 未能有效清除，对 HBV 存在易感性， 为建立 HBV 感染动
物模型提供实验依据， 可用 于研究 HBV 对免疫状态的影响。 |
| 英文摘要: |
| Objective:Nowadays the hepatitis B virus (HBV) animal models have their own limitations, therefore, HBV infection
cannot be studied comprehensively. To establish the HBV bloodbome transmission mouse model will be helpful to the study of the correlation between HBV infection and the immunodeficiency of sucking mice.Methods:Twenty Babl/c suckling mice were divided into
four groups. Each group was injected with the HBV patient serum, health human serum, PBS and nothing, respectively. The temperature
and weight of the mice were recorded and mice serum was collected at 7 d, 1 5 d and 30 d after injection. The enzyme linked immune sorbent assay (ELISA) was carried out to analyze the levels of HBsAg and HBeAg. Meanwhile, the real time, fluorescence, and quantitative
polymerase chain reaction (RT-qPCR) were used to detect the HBV DNA copy in mice serum.Results:The injected mice had the similar
body temperature before. There were no differences with each group's body weight. Four mice of HBV injected group were found with
positive HBsAg, and lasted for 30 days. However, all of them were found with negative HBeAg. The HBV DNA was considered as negative because the DNA titer is lower than 500 IU/mL.Conclusion:The suckling mice of Babl/c can infect HBV by hydrodynamic injection of HBV patients' serum. This study suggests that the Babl/c suckling mouse is susceptibility to HBV due to the immunodeficiency
and disability of eliminating the virus effectively. Our study not only provides the experimental basis for establishing animal model of
HBV infection, but also can be used in the studies of the effect of HBV on immune status. |
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