| 贾文欢 李慧珍 王楠 张琳 辛毅 臧师竹.山药水浸提物对alpha- 葡萄糖苷酶抑制作用的初步研究[J].现代生物医学进展英文版,2015,15(4):645-648. |
| 山药水浸提物对alpha- 葡萄糖苷酶抑制作用的初步研究 |
| The Inhibition Effect of YamExtracts on alpha-glucosidase |
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| DOI： |
| 中文关键词: 山药 alpha- 葡萄糖苷酶 2 型糖尿病 |
| 英文关键词: Yam alpha-glucosidase Type 2 diabetes |
| 基金项目:辽宁省教育厅自然科学一般项目（L2011159） |
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| 中文摘要: |
| 目的：通过研究山药水浸提物对琢-葡萄糖苷酶的抑制作用，初步探索山药对2 型糖尿病的预防和治疗作用。方法：对山药
进行热水浸提，采用1:5 的料液比，浸提温度90 ℃，时间2 小时，超滤获得山药水浸提物，利用alpha- 葡萄糖苷酶对淀粉降解反应建
立酶活检测体系，通过检测酶反应产物分析山药水浸提物对alpha- 葡萄糖苷酶的抑制作用，并计算IC50。结果：山药水浸提物对alpha-葡萄糖苷酶的抑制率大于46 %，IC50值为1.9 g/ml。结论：山药水浸提物对琢- 葡萄糖苷酶的抑制作用提示山药可以作为肥胖或者2 型糖尿病患者有效的一种药食同源食材，本实验结果为2 型糖尿病高危人群及患者合理选择膳食结构提供新的科学依据。 |
| 英文摘要: |
| Objective:To analyze the inhibition effect of yam extracts on alpha-glucosidase and to explore the potential treatment and
prevention effects of yam on type 2 diabetes.Methods:Yam was extracted in hot water using the solid-liquid ratio of 1:5 at temperature
90 ℃for 2 hours. Then the aqueous extracts of yam were acquired after ultrafiltration. An effective 琢-glucosidase activity detection system
was established to detect the inhibition effects of yam extracts according to the degradation of alpha-glucosidase on starch. Finally,
Bioinformatics analysis system was build between interaction of proteins and small molecules. And effective yampolysaccharide components
were screened.Results:Yam extracts can inhibit alpha-glucosidase activity by more than 46%, and the IC50 value was 1.9g/ml.Conclusion:Yam could be used as one of the medicinal and edible plants for type 2 diabetes patients, which provides a new opportunity for
high-risk type 2 diabetes population to choose suitable dietary pattern. |
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