| 付朝霞 汝童 王珊珊 周婷 刘铁夫△.三氧化二砷联合氨氯地平对肝癌HepG2细胞体外作用的实验研究[J].现代生物医学进展英文版,2015,15(4):614-617. |
| 三氧化二砷联合氨氯地平对肝癌HepG2细胞体外作用的实验研究 |
| Experiment of the Effect of Arsenic Trioxide Plus Amlodipine on theProliferation and Apoptosis of Human Liver Cancer HepG-2 Cell in Vitro |
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| DOI： |
| 中文关键词: 氧化二砷 氨氯地平 肝癌细胞 细胞凋亡 细胞周期 |
| 英文关键词: Arsenic trioxide Amlodipine Liver cancer cells Cell apoptosis Cell cycle |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨三氧化二砷与氨氯地平单独用药及联合用药对肝癌HepG2 细胞增殖和凋亡的作用。方法：用不同浓度的三氧化
二砷(4.0、2.0、1.0 umol/L)和氨氯地平(27× 103、18× 103、12× 103 mg/mL)处理体外培养的人肝癌HepG-2 细胞，观察细胞形态的变
化，采用CCK-8 法检测两种药物单独及联合应用对HepG-2 细胞生长增殖的影响，并通过流式细胞术观察其对细胞凋亡及细胞
周期的影响。结果：三氧化二砷和氨氯地平均可以浓度依赖性方式显著增加HepG2 细胞的生长抑制率及其凋亡率，以联合用药的
作用较单独用药更显著(P<0.05)。与三氧化二砷和氨氯地平单独用药相比，联合用药可显著阻滞HepG2 细胞于G2期及S 期。结
论：三氧化二砷和氨氯地平均可显著抑制人肝癌HepG2 细胞的生长和增殖，并促进其凋亡，且二者联合应用时具有协同作用。 |
| 英文摘要: |
| Objective:To explore the effect of arsenic trioxide plus amlodipine on the proliferation and apoptosis of human liver
cancer HepG2 cell line in vitro.Methods:Different concentrations of arsenic trioxide (4.0, 2.0, 1.0 umol/L) and amlodipine (27× 103,
18× 103, 12× 103 mg/mL) were treated on human hepatoma HepG-2 cells in vitro, the changes of cell morphology were observed,
CCK-8 method and flow cytometry were used to test the proliferation and apoptosis of HepG-2 cell respectively.Results:Arsenic trioxideand
amlodipine significantly increased the growth inhibition rate and apoptotic rate of HepG2 cells in a concentration dependent manner,
and the combination of two drugs was more significant(P<0.05). Compared with the arsenic trioxide and amlodipine alone, combination
of two drugs significantly blocked the HepG2 cells in phase G2 and S.Conclusion:Arsenic trioxide and amlodipine could significantly
inhibit the proliferation and apoptosis of human liver cancer HepG2 cell line in vitro, which had a synergistic effect by combination. |
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