| 李玮 赫杰 彭林辉 姚媛菲 史明 李钰.MARVELD1 调控内含子miR-186、miR-335 表达的研究[J].现代生物医学进展英文版,2015,15(3):407-412. |
| MARVELD1 调控内含子miR-186、miR-335 表达的研究 |
| A Research of Intronic miR-186 and miR-335 Regulated byMARVELD1 |
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| DOI： |
| 中文关键词: MARVELD1 miR-186 miR-335 |
| 英文关键词: MARVELD1 miR-186 miR-335 |
| 基金项目:国家自然科学基金项目(31171252) |
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| 中文摘要: |
| 目的：前期研究发现，MARVELD1（具有囊泡运输和膜连接功能的MAL 及相关蛋白1）在多种肿瘤细胞中表达下调，许多
microRNAs也随其发生变化。本研究探讨了MARVELD1 调控miR-186 和miR-335 的表达方式。方法：本研究选取了多种肺癌细
胞系，运用qRT-PCR 的方法检测了MARVELD1 以及miR-186 和miR-335 的表达情况，通过MARVELD1 过表达体系和siRNA
干扰MARVELD1表达的体系分析miR-186 和miR-335 表达变化情况，运用生物信息学网站对miR-186 和miR-335 进行分析，
确认其是否为内含子miRNA，并进一步应用MARVELD1 过表达和RNAi 体系检测MARVELD1 对miR-186 和miR-335 的宿主
基因的影响。结果：在肺癌细胞中，MARVELD1 与miR-186、miR-335 的表达呈现明显的正相关。在过表达MARVELD1 之后，
miR-186、miR-335 出现高表达；当下调MARVELD1 表达时，miR-186、miR-335 则表现低表达。生物信息学分析发现miR-186 和
miR-335 均为内含子miRNA。进一步分析MARVELD1 与miR-186 和miR-335宿主基因的表达关系显示，MARVELD1 可以上
调它们的宿主基因的表达。结论：上述结果表明，MARVELD1 可通过影响内含子miR-186 和miR-335 的宿主基因进而调控二者
的表达，为进一步研究MARVELD1 影响肿瘤细胞的发生机制奠定了基础。 |
| 英文摘要: |
| Objective:It was reported that MARVELD1 downregulated in a variety of tumor cells, but also with its many
microRNAs changes. This study investigated the expression of miR-186 and miR-335 which is regulated by MARVELD1.Methods:First, a variety of lung cancer cell lines were selected for the study, using qRT-PCR method to detect the expression of MARVELD1,
miR-186 and miR-335. Then, the changes of miR-186 and miR-335 expression by MARVELD1 overexpression and MARVELD1
low-expression were analyzed to confirm whether there were intronic miRNA through bioinformatic analysis of miR-186 and miR-335,
and MARVELD1 effects on host genes of miR-186 and miR-335 were detected by using system of MARVELD1 overexpression and
MARVELD1 RNAi.Results:The expression of miR-186 and miR-335 showed a significant positive correlation with MARVELD1 in
lung cancer cells. When overexpressed MARVELD1, miR-186 and miR-335 showed high expression; conversely downregulated
MARVELD1, the expression of miR-186 and miR-335 decreased. Bioinformatics analysis found that miR-186 and miR-335 are intronic
microRNAs. MARVELD1 can upregulate host genes expression of miR-186 or miR-335.Conclusion:These results indicate that
MARVELD1 regulated the expression of intronic miR-186 and miR-335 by regulating their host genes. Our study laid the basis for
further clarification of the mechanismof MARVELD1 affecting tumor cells. |
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