| 姚蜜蜜 周进 李庆赟 吕浩 蔡中华.浮游细菌群落变化对锥状斯氏藻藻华的影响[J].现代生物医学进展英文版,2015,15(2):201-207. |
| 浮游细菌群落变化对锥状斯氏藻藻华的影响 |
| Dynamics of Bacterial Community during the Bloom Caused byScrippsiella Trochoidea |
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| DOI： |
| 中文关键词: 锥状斯氏藻 浮游细菌群落 硫化物形态 藻菌关系 |
| 英文关键词: Bacterial community Sulfide form Relationship between algae and bacteria |
| 基金项目:科技部 973 计划前期专项(2012CB426504)；国家海洋公益性项目 (201 2050209,201305021 1)；
深圳市基础研究计划（ JCYJ201 30402145002375）；南山区创新研发基金（ KC2013JSCX003A） |
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| 中文摘要: |
| 目 的： 本实验以大亚湾原发性锥状斯氏藻为例，采集野外样品， 分析藻华不同时期主要菌群的结构和环境样品的硫化物与赋
存形态。 方法： 应用末端限制性片 段长度多态性技术和主成分分析方法， 分析藻华发生过程中浮游细菌群落相似程度的情况，得
到差异显著的浮游细菌类群。 选出代表类群的样品进行 16S rDNA 高变区测序， 获取浮游细菌的分类结果及相对丰度。 采用
Pearson 相关性分析浮游细菌、 藻、 硫化物两两间 的相互关系 。 结果： 早期藻华以 为主导， 各优势菌群
（ ： ） 的 比 例 约 为 8:1 :21， 硫 元 素 主 要 以 DMS 形 式 存 在 ； 而 后 期
成为优势物种， 各优势菌群的比例 转变为 3:5:25， 硫的赋存形态由 DMS 转变为 DMSO； 在藻
华的前期与 后期均以优势种存在。 本实验还发现藻华不同 时期 4 种与藻呈正向相关的细菌，以及 8 种对藻起负向调节作用的细
菌， 它们在藻华生消的过程中扮演着不同的角色。 结论： 菌群的组成性改变与 藻华生消具有一定的相关性， 并对藻类的硫代谢产
生影响。结果的获得有助于认识藻华微生物学过程中硫代谢的生态学功能， 拓展锥状斯氏藻藻华的理论认识。 |
| 英文摘要: |
| Objective:This experiment focuses on the bloom in Daya Bay to explore the microbial
community structure and the occurrence form of sulfide in different bloom stages.Methods:Bacterial community structures of the bloom
were evaluated by T-RFLP and PCA. The genetic diversity of bacterial community was analyzed by illumina sequencing. Pearson
correlation coefficient was used to describe the correlation among bacterial community, algae and sulfur.Results:In early stage of the
bloom, the ratio of advantage bacterium groups ( ) was about 8:1:21 , and
played the leading role. Sulfur existed mainly in the form of DMS. In decline period of the bloom, the proportion of
each advantage bacterium group turned into 3:5:25, and became the dominant species. The combined form of sulfur
changed into DMSO. And was the permanent dominant species during the bloom. In addition, this study found that
there were kinds of bacteria closely related to in different periods of the bloom, including four positively regulated
bacteria and eight negatively regulated bacteria, which played different roles during the bloom.Conclusion:It is confirmed that changes
in composition of bacterial flora have relations with algal bloom. And dynamics of bacterial community also has effect on the sulfur
metabolism. Therefore, this work is helpful to understand the microbiology ecology function of sulfur metabolism during the bloom, and
to expand theoretical knowledge of bloom. |
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