| Objective:The aim of this study was to determine the allele frequencies and population genetic parameters of 18 STR
(short tandem repeat) loci (D5S818, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, vWA, D8S1179, D16S539, Penta E, TPOX,
TH01, D19S433, D18S51, FGA, D6S1043,D13S317, and D12S391) in Jiajiang county of Sichuan province and to calculate the technical
parameters of the DNATyperTM19 kit.Methods:PCR amplification using blood as template directly and capillary electrophoresis technologies
were employed to determine the genotypes of 18 STR loci for 226 individuals. PowerStatsV12 software was used for analysis
and statistics.Results:202 alleles were recognized, with frequencies ranging from 0.002 to 0.527. No departures from Hardy-Weinberg
expectations were detected for all 18 loci studied (P>0.05). The statistical analysis of 18 STR loci showed the heterozygosity ≥ 0.633, the
matching probability ≥ 0.018, the discrimination power ≥ 0.790, the polymorphic information content ≥ 0.56, the probability of paternity
exclusion ≥ 0.332, and the typical paternity index ≥ 1.36.Conclusion:This work studied the genetic polymorphism of 18 short tandem
repeat loci in Jiajiang county of Sichuan province providing pronounced basic data for subsequent human population genetic and
forensic DNA research work. The cumulative matching probability of the DNATyperTM19 kit reaches 3.477× 10-22, while its cumulative
excluding probability of paternity is 0.999999974. |
