| 林铿强 石琴 何峰 陈群△ 许德新.非小细胞肺癌组织中miR-19b 与miR-20a 的表达及临床意义[J].现代生物医学进展英文版,2014,14(36):7073-7075. |
| 非小细胞肺癌组织中miR-19b 与miR-20a 的表达及临床意义 |
| Expression and Clinical Significance of miR-19b and miR-20a in Non-SmallCell Lung Cancer |
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| DOI： |
| 中文关键词: miR-19b miR-20a 非小细胞肺癌 Real-time RT-PCR |
| 英文关键词: miR-19b miR-20a Non-small cell lung cancer Real-time RT-PCR |
| 基金项目:福州市卫生局科技计划项目（榕卫人[2013]150号） |
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| 中文摘要: |
| 目的：检测miR-19b 与miR-20a 在非小细胞肺癌（NSCLC）组织中的表达，探讨两者与肺癌临床病理的关系。方法：选择我院
NSCLC 肺癌患者50 例，使用Real-time RT-PCR 法对其癌组织及癌旁正常组织中miR-19b 和miR-20a 的含量进行检测，并利用2
（-△△CT)法处理结果，分析与临床病理资料的关系。结果：相对于内参U6，miR-19b 基因在NSCLC 组织中的表达量明显低于癌
旁正常组织，而miR-20a 基因在NSCLC 组织中的表达量明显高于癌旁正常组织，差异具有统计学意义（P<0.05）；在NSCLC 组织
标本中，miR-19b 基因表达量在临床分期I-II的标本中明显高于临床分期III，而miR-20a 基因表达量在临床分期I-II的标本中明
显低于临床分期III，差异均就有统计学意义（P<0.05）；miR-19b 基因表达量在肿瘤病理低分化组织中明显低于肿瘤病理高分化组
织，而miR-20a 基因表达量在肿瘤病理低分化组织中明显高于肿瘤病理高分化组织，差异均具有统计学意义（均P<0.05）。结论：
miR-19b 低表达和miR-20a高表达与NSCLC 的临床分期、病理分级具有密切关系，早期检测有助于NSCLC诊断和治疗。 |
| 英文摘要: |
| Objective:To detect the expressions of miR-19b and miR-20a in the tissues of non-small cell lung cancer (NSCLC)
and to explore the relationships between the expressions of miR-19b and miR-20a and the pathology.Methods:50 patients with NSCLC
in our hospital were selected, and 2（-△△CT) method was used for quantitative analysis on the relationships of the expressions of
miR-19b and miR-20a in the tissues of the carcinoma and the adjacent normal lung with the pathological characteristics.Results:With
the reference of U6, the expression of miR-19b in NSCLC tissues was lower than that of the adjacent normal tissues, while the expression
of miR-20a in NSCLC tissues was higher than that of the adjacent normal tissues, and the differences were statistically significant (P<0.
05); the expression of miR-19b in the I-II clinical stage was higher than that of the III, while the expression of miR-20a in the I-II clinical
stage was lower than that of the III, and the differences were statistically significant (P<0.05); the expression of miR-19b in the low
pathological grade was lower than that of the high pathological grade, while the expression of miR-20a was higher, and the differences
were statistically significant (P<0.05).Conclusion:It is indicated that the low expression of miR-19b and the high expression of miR-20a
should be closely related to the clinical stages and the pathological types of NSCLC, which would be helpful to the diagnosis and
treatment of NSCLC by early detection. |
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