| 王英 石彦1 施明 张敏 王洪波
孙艳玲 苏海滨 金磊 刘振文 王福生.肝移植急性排斥反应时外周血Th17细胞的特征及意义[J].现代生物医学进展英文版,2014,14(36):7030-7033. |
| 肝移植急性排斥反应时外周血Th17细胞的特征及意义 |
| Characteristics of Th17 Cells in Peripheral Blood in Liver Allograft Recipientswith Acute Rejection |
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| DOI： |
| 中文关键词: 肝移植 Th17细胞 急性排斥反应 |
| 英文关键词: liver transplantation Th17 cells Acute rejection |
| 基金项目:国家"863" 课题（2013AA020102）；全军医学科技" 十二五" 科研项目重点课题（BWS11J075(11-15)）；
首都临床特色应用研究课题（Z111107058811069） |
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| 中文摘要: |
| 目的：观测肝移植患者术前及术后急性排斥时外周血中Th17细胞频率变化特征，探讨其与肝移植术后急性排斥发生的关系。
方法：对18例于我院进行肝移植手术的患者进行随访研究，应用流式细胞仪检测各随访点外周血中Th17细胞频率。随访期间发生
急性排斥反应的患者为急排组（n=7，男女比为5/2），未发生急性排斥反应者为非急排组（n=11，男女比为8/3），比较两组患者肝移植
前后外周血中Th17细胞频率的变化规律。结果：急排组与非急排组患者肝移植术前外周血中Th17 细胞频率无统计学差异（P=0.
672），而急排组患者发生急性排斥反应时与非急排组患者相比Th17细胞频率明显升高（P=0.002）。在随访研究中，急排组患者在发
生急性排斥反应时外周血中Th17 细胞频率较未发生急性排斥反应时增高，且Th17 细胞频率与ALT变化趋势一致，呈明显正性相
关。而非急排组患者术后随访期间外周血中Th17 细胞频率无统计学差异。结论：肝移植患者术后发生急性排斥反应时外周血中
Th17细胞频率明显升高，变化趋势与ALT水平一致，呈正性相关，可能成为诊断急性排斥反应的潜在指标。 |
| 英文摘要: |
| Objective:To investigate the characteristics of Th17 cells in peripheral blood in liver transplant patients, and its
association with acute rejection (AR).Methods:18 patients who underwent liver transplantation in our hospital were enrolled for this study.
Peripheral blood was obtained fromthese participants longitudinally: pretransplantation, posttransplantation within 1 year's follow up, and
at the time of an episode of AR to measure the frequencies of circulating Th17 cells by flow cytometry. All participants were divided into
two groups: acute rejection group who suffered an AR (n=7, male/female is 5/2) during 1 year's follow up after liver transplanttation, and
non-rejection group who were in stable liver function (n=11, male/female is 8/3). The characteristics of circulating Th17 cells between the 2
groups were analyzed.Results:There were no significant difference in the levels of circulating Th17 cells in pretransplantation (P=0.672).
However, the frequency of circulating Th17 cells were significantly higher in acute rejection group as compared with the non-rejection
group (P=0.002). Also, the longitudinal analysis revealed that the frequency of circulating Th17 cells dramatically increased at the time in
acute rejection occurrence as compared with pretransplantation (P=0.007). In addition, we found that the variation tendency of frequency of
circulating Th17 cells was similiar with the level of ALT. The frequency of Th17 cells was maintained stable level in non-rejection group
during the follow up period.Conclusion:The frequency of Th17 cells in peripheral blood significantly increased in AR with a similar
variation tendency for the ALTlevel. Itwould be potentially acting as a marker for AR diagnosis in liver transplantation. |
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