| 王倩 张娟 周一 罗帅寒天 杨涛 李昌琪 毕方方.CFA注射致炎性疼痛过程中海马内BDNF的表达及作用研究[J].现代生物医学进展英文版,2014,14(36):7001-7006. |
| CFA注射致炎性疼痛过程中海马内BDNF的表达及作用研究 |
| The Expression and Role of BDNF in the Hippocampus during InflammatoryPain after CFA Administration* |
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| DOI： |
| 中文关键词: 炎性痛 抑郁样行为 海马 脑源性神经营养因子 |
| 英文关键词: Inflammatory pain Depression-like behavior Hippocampus Brain-derived neurotrophic factor |
| 基金项目:国家自然科学基金项目(81100908)；国家级大学生创新课题（AE1153） |
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| 中文摘要: |
| 目的：探索完全弗氏佐剂（Complete Freund’s Adjuvant，CFA）致炎性疼痛后大鼠海马内脑源性神经营养因子（brain-derived
neurotrophic factor，BDNF)表达的变化及其作用。方法：雄性SD 大鼠随机分为溶媒对照组和实验组,实验组大鼠左侧足底皮下注
射CFA 和生理盐水混合溶剂100 滋L，建立炎性疼痛模型，注射后大鼠分别存活1 天、4天、7 天、14 天（n=20 只/ 组）。在建模前和
建模后不同时间点采用Von Frey纤维检测大鼠50%机械缩足阈值（paw withdrawal threshold，PWT）的变化。采用强迫游泳实验和
糖水偏好实验检测大鼠抑郁样行为，采用RT-PCR检测不同时间点大鼠海马中BDNF mRNA的水平，采用ELISA和免疫组织化
学法检测BDNF的表达变化。结果：足底注射CFA 后1 天50%PWT 下降,持续至14 天仍低于基础值。在强迫游泳实验中，注射
CFA7 天后的大鼠不动时间百分比增加，一直持续到CFA 注射后14天。糖水偏好实验中注射CFA 后7 天组、14 天组大鼠表现出
对糖水偏好的降低，提示注射CFA 后7 天大鼠出现抑郁样行为并持续到第14天。在足底注射CFA 第7 天后BDNF mRNA和
BDNF的水平达到高峰，第14 天表达下调并基本恢复正常水平。结论：结果提示，足底注射CFA能诱导大鼠产生炎性痛，CFA 注
射7 天后出现抑郁样行为，此时海马内BDNF mRNA和BDNF蛋白水平均上调，可能与足底注射CFA 后出现的抑郁样行为的发
生密切相关。 |
| 英文摘要: |
| Objective:To investigate the expression and role of brain-derived neurotrophic factor (BDNF) in hippocampus of rats
followed by Complete Freund’s Adjuvant (CFA) induced inflammatory pain.Methods:Adult male Sprague-Dawley rats were randomly
divided into control group and experimental group. The inflammatory pain models were established by subcutaneously injecting mixture
of CFA and saline into the left plantar of the rats. The experimental rats were survived 1 day, 4 days, 7 days, 14 days (N=20/group) after
injection of CFA respectively. The alterations of 50% paw withdrawal threshold (PWT) were measured at different time points
respectively before and after CFA injection with Von Frey Fibres. The depression-like behaviors tested with sucrose preference test and
forced swimming test. While the level of BDNF mRNA at different time points was detected by RT-PCR and the expression of BDNF in
the hippocampus was detected by immunohistochemistry and ELISA.Results:The results showed that the 50% PWT decreased at 1d
after CFA injection and sustained, but was still lower than baseline at 14d. The percentage of immobility time increased at 7d and 14d
after CFA administration. Rats had no sucrose preference on 7d and 14d after CFA injection, which indicated that rats exhibited visible
depression-like behaviors at 7d and maintained to 14d. The level of BDNF mRNA and BDNF gradually increased after CFA injection,
and reached the peak on 7d after CFA injection, then decreased on 14d after CFA injection. There was no difference between the two
groups.Conclusion:The present study suggested that the upregulation of BDNF mRNA and BDNF in the hippocampus might be
responsible for depression-like behaviors induced by CFA administration. |
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