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余震余州吴骐而石定岳毅刚彭湃.不同剂量链尿佐菌素诱导 2 型糖尿病大鼠模型的研究[J].现代生物医学进展英文版,2014,14(35):6835-6838.

不同剂量链尿佐菌素诱导 2 型糖尿病大鼠模型的研究

Study on Type 2 Diabetic Rat Models induced by Different Dosesof Streptozotocin

DOI：

中文关键词: 2 型糖尿病动物模型链脲佐菌素

英文关键词: Type 2 diabetes mellitus Animal model Streptozotocin

基金项目:国家自然科学基金面上项目（ 81 272118）；国家自然科学基金面上项目（ 30973752）

Author Name	Affiliation
YU Zhen, YU Zhou, WU JI-er, SHI Ding, YUE YI-gang, PENG Pai	桂林医学院附属医院烧伤整形美容科；第四军医大学西京医院全军整形外科研究所

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中文摘要:

目的：探讨不同剂量链脲佐菌素(Streptozotocin， STZ)联合高糖高脂饮食对 2 型糖尿病大鼠模型建立的影响。方法： 90 只 8 周龄 SD 雄性大鼠随机平均分为六组：普通饲料喂养 + 缓冲液组、高糖高脂饲料喂养 + 缓冲液（ H.E 组）、高糖高脂饲料喂养 +35 mg/kg 链尿佐菌素组（ H.E+35 mg/kg STZ 组）、高糖高脂饲料喂养 +45 mg/kg 链尿佐菌素组（ H.E+45 mg/kg STZ 组）、高糖高脂饲料喂养 +55 mg/kg 链尿佐菌素组（ H.E+55 mg/kg STZ 组）及高糖高脂饲料喂养 +65 mg/kg 链尿佐菌素组(H.E+65 mg/kg STZ 组)，高糖高脂饲料喂养 4 周后诱导胰岛素抵抗，继之腹腔注射 STZ，建立 2 型糖尿病大鼠模型。检测体重、胰岛素、空腹血糖、血脂、胰岛素敏感指数（ ISI）。结果：与常规饮食组相比，高糖高脂饮食各组大鼠出现空腹血浆胰岛素（ FINS）、空腹血糖（ FBG）、血清甘油三脂（ TG）、总胆固醇（ TC）、游离脂肪酸（ FFA）显著升高（ P<0.01 ）， ISI 显著下降（ P<0.01）。不同剂量 STZ 注射， H.E+45 mg/kg STZ 组成模率最高且无自愈现象。结论：通过 STZ 腹腔注射联合高糖高脂饮食可成功复制出实验性 2 型糖尿病动物模型， 45 mg/kg 为 STZ 理想注射剂量。

英文摘要:

Objective:To investigate the effects of different doses of streptozotocin and high-glucose-high-fat diet on the establishment of type 2 diabetic rat model.Methods:Ninety 8-month-old SD male rats were randomly divided into six groups： Normal feeding group, high-glucose-high-fat feeding group (H.E group), and high-glucose-high-fat+STZ group which were intraperitoneal injected STZ with a dose of 35 mg/kg, 45 mg/kg, 55 mg/kg, and 65 mg/kg, respectively (H.E+35/45/55/65 mg/kg_STZ group). High-glucosehigh-fat diet feeding for four weeks to induce insulin resistance, then streptozotion was injected intraperitoneally to establish the type 2 diabetes mellitus model. The changes of body weight, blood insulin level, fasting blood glucose level, blood lipid level, and insulin sensitivity index (ISI) were measured and statistically analyzed.Results:Compared with the control group, the fasting plasma insulin, fasting blood glucose, serum triglyceride (TG), total cholesterol (TC) and free fatty acids (FFA) of H.E group and H.E ＋ STZ group significantly increased (P<0.01 ) and the ISI significantly decreased (P<0.01 ). From different doses of STZ injection, the 45 mg/kg STZ injection had the highest successful rate and effective stability to establish the models.Conclusion:High-glucose-high-fat diet combined with STZ injection can successfully replicate the experimental animal models of type 2 diabetes, and 45 mg/kg is an ideal dose for STZ injection.

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