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目的：探讨cAMP 反应原件结合蛋白（cyclic AMP response element-binding protein，CREB）和CXC 趋化因子受体2（CXC chemokine receptor 2，CXCR2）在非小细胞肺癌（non-small cell lung cancer，NSCLC）组织中的表达及与肺癌发生、发展的关系。方法：采用免疫组化Elivision 法检测CREB 和CXCR2 在49 例NSCLC组织中的表达，同时随机选取20 例癌旁正常肺组织作为对照。结果：NSCLC 中CREB 和CXCR2 的表达阳性率分别为57.1 %和69.4 %，均显著高于癌旁正常组织中的10.0 %和15.0% （P＜0.05）；同时，CREB 和CXCR2 在NSCLC组织中的表达不仅与肿瘤的TNM 分期有关（P＜0.05），而且还与肿瘤的分化程度有关（P＜0.05）；另外，CREB 在吸烟患者中的表达明显高于非吸烟患者（P＜0.05），在鳞癌组织中的表达明显高于腺癌（P＜0.05）； CREB 和CXCR2 的表达呈正相关。结论：CREB 和CXCR2 可能参与了NSCLC的发生和发展，为NSCLC 的靶向治疗提供了新的依据。

英文摘要:

Objective:To investigate the protein expression of cyclic AMP response element-binding protein (CREB) and CXC chemokine receptor 2 (CXCR2) in non-small cell lung cancer (NSCLC) in order to investigate their relationship with clinical and pathological features of NSCLC patients.Methods:The immunohistochemical Elivision method was used to detect the expression of CREB and CXCR2 in NSCLC tissue(n=49) and adjacent normal lung tissue(n=20).Results:High expression of CREB and CXCR2 was observed in 57.1%and 69.4%of NSCLC tissue and 10%and 15%of adjacent normal lung tissue (P＜0.05). The expression of CREB and CXCR2 was related to both tumor stages and cell differentiation in NSCLC (P＜0.05). CREB expression was obviously higher in both squamous cell carcinoma and the smokings than that in adenocarcinoma and non-smokings (P＜0.05), whereas CXCR2 in both histologic subtype and Smoking status was less or not affected by them (P＞0.05). CREB expression was demonstrated to associate positively with CXCR2.Conclusion:CREB and CXCR2 may play a vital synergetic role in the progress of NSCLC because of overexpression in NSCLC tissue, which may provide a potential therapeutic target for the prevention and treatment of NSCLC.

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