| 唐艺丹 周丽曼 于文功 宫倩红.一株海洋真菌Penicillium sp.QF046的细菌群体感应抑制剂的分离和鉴定[J].现代生物医学进展英文版,2014,14(34):6607-6610. |
| 一株海洋真菌Penicillium sp.QF046的细菌群体感应抑制剂的分离和鉴定 |
| Isolation and Identification of QuorumSensing Inhibitors from a Strain of Marine Fungus Penicillium sp.QF046 |
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| DOI： |
| 中文关键词: 紫色杆菌CV026 海洋真菌 群体感应 紫色菌素 星形曲霉毒素 |
| 英文关键词: Chromobacterium violaceum CV026 Marine fungi Quorumsensing Violacein Asteltoxin |
| 基金项目:国家自然科学基金项目（81102368）；留学归国人员科研启动金教外司留（[
2013]639） |
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| 中文摘要: |
| 目的：从海洋真菌中筛选得到新型群体感应抑制剂，并对其进行活性评价。方法：首先利用紫色杆菌CV026 指示菌株对真菌
发酵粗提物进行活性筛选。其次通过18S rDNA 序列比对进行菌种鉴定，同时采用硅胶柱色谱、凝胶柱色谱和高效液相色谱等技术并结合活性追踪检测分离纯化的活性化合物，再通过核磁质谱分析确定其结构。最后利用定量测定方法检测其在亚抑菌浓度下对紫色杆菌紫色菌素产量影响以及RT-PCR 检测与QS 调控相关基因的mRNA 表达的影响。结果：从海藻共生菌中筛选到一株具有紫色杆菌群体感应抑制活性的海洋真菌Penicillium sp.QF046，其次级代谢产物中纯化到的活性化合物根据结构鉴定为一种星形曲霉毒素（asteltoxin）。该化合物对于紫色杆菌群体感应抑制浓度低于阳性对照化合物呋喃酮C30，同时抑制了群体感应相
关基因mRNA 水平的表达。结论：从海洋真菌Penicillium sp.QF046代谢产物中发现了一种抑制紫色杆菌群体感应的星形曲霉毒素，为进一步通过结构改造研发新型抗菌药物提供良好的前体化合物。 |
| 英文摘要: |
| Objective:The purpose of this article is to find new quorumsensing inhibitors (QSI) fromstrains of marine fungus and
analyze the inhibitory activity.Methods:Quorum sensing inhibitory activity of crude extracting from this strain was monitored by Chromobacterium violacceum CV026
and the purpose strain was identified based on phylogenetic analysis of 18S rDNA sequences. The
bioactivator with anti-QS activity was separated by bioactivity-guided fractionation from the crude extract, using silica-gel column
chromatography, Sephadex LH-20 gel column chromatography and HPLC, and was identified based on spectroscopic analysis. Its QS
inhibitory activity was measured by quantifying QS-controlled violacein production, and the effect of mRNA of QS-regulated gene was
measured by RT-PCR.Results:A marine fungus strain Penicilliu sp.QF046from seaweed symbiotic was found to have significant C.
violaceum QS inhibitory activity. The QSI in the secondary metabolites was purified and identified as a kind of asteltoxin. It significantly
reduced violacein production in a lower concentration than furanone C30 and the mRNA level of QS-related genes, indicating inhibition
of QS.Conclusion:An asteltoxin from marine fungi strain Penicillium Sp.QF046 can inhibit QS in C. violaceum, and could be a
precursor compound for further study of novel antibacterial based on structural modification. |
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