| 施伟杰 曾玉 姚纯 曹笑梅 童华.miR-29a靶基因预测及其相关生物信息学分析[J].现代生物医学进展英文版,2014,14(32):6340-6344. |
| miR-29a靶基因预测及其相关生物信息学分析 |
| miR-29a Target Genes Prediction and Related Bioinformatics Analysis |
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| DOI： |
| 中文关键词: miR-29a 靶基因 基因本体 信号通路 生物信息学 |
| 英文关键词: MiRNA-29a Target genes Gene ontology Pathway Bioinformatics |
| 基金项目:国家自然科学基金项目（81300654） |
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| 中文摘要: |
| 目的：通过对miR-29a 进行靶基因预测及相关生物信息学分析，为miR-29a 靶基因的实验验证提供数据支持，以期为深入研
究miR-29a 的生物学功能和调控机制提供理论指导。方法：利用PubMed 检索miR-29a 相关文章，通过miRBase 在线工具分析
miR-29a 序列。应用TargetScan 及miRNAda 两种计算方法预测miR-29a 靶基因并取其交集作为分析的基因集合，分别进行基因
本体（gene ontology, GO）中的分子功能和生物学过程以及KEGG（Kyoto Encyclopedia of Genes and Genomes）生物通路富集分析。
结果：（1）miR-29a 序列在多物种间具有高度保守性。（2）两种方法预测miR-29a 靶基因交集共191个。（3）miR-29a 靶基因GO分
子功能集中于转录因子活性、DNA结合和钙离子结合等（P<0.05)；miR-29a 靶基因GO生物学过程集中于调控转录、细胞粘附、细
胞增殖与凋亡等（P<0.05)；KEGG 生物通路主要富集于PI3K-AKT 信号通路、JAK-STAT 信号通路、T 细胞受体信号通路和胰岛素
信号通路等信号转导通路，以及肺小细胞癌和子宫内膜癌等疾病通路（P<0.05）。结论：miR-29a 可能通过参与多个靶基因信号通
路的调控，在机体的多种生理病理过程中发挥重要作用，是一个颇有研究价值的生物学靶标。 |
| 英文摘要: |
| Objective:Through the miR-29a target genes prediction and related bioinformatics analysis, the data provide
experimental validation and theoretical guidance for further investigation of miR-29a related biological function and regulatory
mechanisms.Methods:The studies of miR-29a were reviewed by Pubmed, and the sequence of miR-29a was obtained from miRBase.
Target Scan and miRNAda were used to predict target genes of miR-29a, and the intersection of the two results was performed by Gene
Ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis.Results:(1) MiR-29a sequences were
highly conserved in various species. (2) There were 191 predicted target genes in the gene set. (3) GO analyses of miR-29a target genes
were enriched in transcription factor activity, DNA binding and calcium ion binding (GO molecular function, P<0.05), and enriched in
regulation of transcription process, cell adhesion, cell proliferation and apoptosis (GO biology process, P<0.05); In KEGG pathway, the
gene set mainly located in PI3K-AKT signaling pathway, JAK-STAT signaling pathway, T cell receptor signaling pathway and insulin
signaling pathway (signal transduction pathway, P<0.05), small cell lung cancer and Endometrial cancer etc (human diseases pathway,
P<0.05).Conclusion:miR-29a may be involved in the different signaling pathways via regulation of varies target genes and play an
important role in the physiological and pathological processes. It is a valuable target for further investigation. |
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