| 于磊 孔艺蓉 孙维胜 秦璞 冯金环.NF-kB 在哮喘病患儿支气管肺泡灌洗液中的表达及意义[J].现代生物医学进展英文版,2014,14(32):6296-6299. |
| NF-kB 在哮喘病患儿支气管肺泡灌洗液中的表达及意义 |
| A New Regulation Pathway in Asthma: NF-kB Activates theExpression of IL-17 |
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| DOI： |
| 中文关键词: 支气管哮喘 儿童 NF-kB 发病机理 |
| 英文关键词: Bronchial asthma Children NF- kB Pathogenesis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨NF-kB、IL-17 在儿童支气管哮喘中的作用及机制。本文通过观察哮喘急性发作儿童支气管肺泡灌洗液(BALF)
NF-kB、IL-17 的水平变化,探讨其在哮喘急性发作儿童气道炎症中的作用。方法：我院2012 年12 月-2013 年2 月期间行纤维支气管镜检查患儿共80 例,包括哮喘急性发作组(哮喘组,n=40)、非喘息组(肺炎组,n=20)及对照组(n=20)，收集所有病例的BALF,进行细胞学分类，RT-PCR 法测定BALF中细胞中NF-kB 蛋白（P65mRNA）、IL-17mRNA的表达；Western 法检测P65 蛋白、IL-17 蛋
白表达。结果：与对照组比较，哮喘组和肺炎组患儿的P65mRNA、IL-17mRNA 水平均明显增高；P65 蛋白、IL-17 蛋白水平均明显
增高(均P＜0.05)；哮喘组患儿的P65mRNA、IL-17mRNA水平、P65 蛋白、IL-17 蛋白水平较肺炎组高(P＜0.05)。结论：NF-kB、IL-17
在哮喘儿童气道炎症中发挥重要作用, NF-kB 通过调控IL-17 来实现促进哮喘病情进展。 |
| 英文摘要: |
| Objective:To investigate the role of NF-kB and IL-17 on the etiology of asthma and to explore the potential relationships
between them.Methods:Bronchoalveolar lavage fluid (BALF) was obtained by fiberoptic bronchoscope setting into the trachea of
asthmatic patients (n=40), healthy individuals (n=20) and non-asthma patients(n=20). The expressions of NF-kB (P65) mRNA and IL-17
mRNA were qualified through the RT-PCR and Western blotting.Results:The distribution of expression of P65 mRNA was significantly
different between group1-asthma group (acute attack), group 2-non-asthma group and healthy controls. The relative capacity of P65 and
IL-17 in asthma group was obviously higher than those of other groups.Conclusion:It is indicated that the IL-17 and NF-kB might be
involved in the etiology of asthma, especially for the acute one. Our study also suggested a potential cause-effect association between
IL-17 and NF-kB, which is the first investigation of the relationship between the two factors. |
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