| 靳晓芳 郭琪 李蓓 马宝玉 王晨.姜黄素对小胶质细胞的活化的影响[J].现代生物医学进展英文版,2014,14(32):6257-6259. |
| 姜黄素对小胶质细胞的活化的影响 |
| The Effect of Curcumin on Microglial Activation |
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| DOI： |
| 中文关键词: 小胶质细胞 姜黄素 炎症 TOLR4 脂多糖 |
| 英文关键词: Microglia Curcumin Inflammation TOLR4 Lipopolysaccharide |
| 基金项目:国家自然科学基金项目（81171237） |
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| 中文摘要: |
| 目的：研究姜黄素对脂多糖引起的小胶质细胞活化状态的影响，并探讨TOLR4受体在其中的作用。方法：采用体外培养N9
小鼠小胶质细胞系，脂多糖作为刺激，激活小胶质细胞。采用ELISA法检测小胶质细胞培养基中TNF-alpha、IL-1beta和IL-6 的含量；相差显微镜观测细胞形态；免疫细胞化学和western blot 观测小胶质细胞TOLR4 受体表达情况。结果：与对照组相比，暴露于100ng/ml 脂多糖24 h 的小胶质细胞促炎症因子TNF-alpha、IL1beta和IL-6 释放量明显增加(P<0.05)，姜黄素浓度为10 uM时，可显著减少小胶质细胞释放TNF-alpha、IL-1beta和IL-6(P<0.05)，此外，姜黄素还可改善小胶质细胞形态，并降低暴露于脂多糖中的小胶质细胞
TOLR4受体的表达。结论：姜黄素可能通过抑制TOLR4 表达，减轻了脂多糖对小胶质细胞的激活。 |
| 英文摘要: |
| Objective:To investigate the effect of curcumin (Cur) on lipopolysaccharide (LPS)-induced microglial activation and
the role of Toller Receptor 4 (TOLR4) in the effect.Methods:N9 microglia cell line exposed to LPS for 24 h was used to mimic the model
of microglial inflammation. ELISA was used to assess the concentrations of tumor necrosis factor alpha(TNF-alpha), interleukin (IL)-1beta and
IL-6. Phase contrast microscope was used to record the morphology of microglial cells exposed to LPS. Immunocytochemistry and western
blot were used to measure the expression of TOLR4.Results:Compared with that in the control, LPS of 100 ng/ml increased the concentrations
of TNF-alpha, IL-1beta and IL-6 in the supernatant (P<0.05), while curcumin of 10 uM decreased the levels of TNF-alpha, IL-1beta and
IL-6 markedly (P<0.05). In addition, curcumin ameliorated the morphology and decreased the expression of TOLR4 in mi- croglial cells
exposed to LPS.Conclusion:Curcumin attenuated microglial activation induced by LPS, and the inhibition of TOLR4 may be associated
with the effect. |
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