| 杨春艳 李书书 王姝 林梓桐 刘澍 王超 王铁梅.1-alpha羟化酶和人成纤维细胞生长因子-23 基因修饰小鼠离体股骨及下颌
骨X线影像的分形分析研究[J].现代生物医学进展英文版,2014,14(32):6229-6232. |
| 1-alpha羟化酶和人成纤维细胞生长因子-23 基因修饰小鼠离体股骨及下颌
骨X线影像的分形分析研究 |
| Fractal Analysis of X-ray Photography to Isolated Femora and Mandible inMice GeneticallyModified by 1-alpha-Hydroxylase and Human FibroblastGrowth Factor-23 |
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| DOI： |
| 中文关键词: ：小鼠 1-alpha羟化酶 人成纤维细胞生长因子-23 骨质疏松症 X 线影像 分形分析 |
| 英文关键词: Mice 1-alpha-hydroxylase Human fibroblast growth factor-23 Osteoporosis X-ray photography Fractal analysis |
| 基金项目: |
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| 中文摘要: |
| 目的：初步分析1-alpha羟化酶（1-alpha-hydroxylase，1alpha(OH)ase）、人成纤维细胞生长因子-23（fibroblast growth factor-23，
FGF23）基因修饰小鼠离体股骨及下颌骨X 线平片的分形维数（fractal dimension, FD），为其在骨质疏松症评价中的运用提供实验
依据。方法：以6 周龄的1alpha(OH)ase-/-、FGF23+、1alpha(OH)ase-/-FGF23+小鼠为模型，以同龄的野生型（Wild-Type, WT）小鼠为对照，采集
小鼠的股骨和下颌骨，使用离体X-射线摄影评估骨质疏松状况，利用Image J软件测量各组小鼠股骨和下颌骨X- 射线影像兴趣
区域的FD 值，分析其差异及相关性。结果：1）与WT 小鼠相比，1alpha(OH)ase-/-、FGF23+、1琢(OH)ase-/-FGF23+小鼠均出现骨质疏松征
象，包括股骨和下颌骨骨长度及体积减少、骨密度明显下降，下颌磨牙、切牙及牙槽骨的射线透光度增高、牙髓腔宽大、根管变薄。
2）1琢(OH)ase-/-、FGF23+、1琢(OH)ase-/-FGF23+小鼠的股骨头和股骨远端干骺端的FD 值均较WT 小鼠明显增加（P<0.05），但股骨腔
未见差异；下颌骨升支的FD 值在1琢(OH)ase-/-、FGF23+、1琢(OH)ase-/-FGF23+小鼠中明显增高（P<0.05），而在牙槽骨中未见差异；3）
下颌骨升支X 平片的FD 值与股骨干骺端的FD值显著相关（r=0.541，P=0.049）。结论：FD 值可以较好地反映骨质疏松状况，股骨
干骺端、下颌骨升支是较合适的兴趣部位，分形分析可运用于下颌骨影像学的再分析。 |
| 英文摘要: |
| Objective:The fractal dimension (FD) of X-ray photography to the isolated femur and mandible were explored in
mice genetically modified by 1-alpha-hydroxylase (1alpha(OH)ase) /or human fibroblast growth factor-23 (FGF23), which tried to provide
an experimental evidence for its application in the diagnosis of osteoporosis.Methods:Six weeks old 1alpha(OH)ase-/-, FGF23+ and 1alpha(OH)
ase-/-FGF23+mice were used as the model of osteoporosis, and their wild type (WT) littermates were used as control. The isolated femur,
mandible microarchitecture and the status of osteoporosis were estimated by X-ray photography. In addition, the FD values on the interest
region in X-ray photography were measured by Image J software, which was then used to analyze the difference of FD value between the
models and control, and the relationship between the FD in the femur and mandible as well.Results:1) Compared with WT mice, all the
1琢(OH)ase-/-, FGF23+ and 1琢(OH)ase-/-FGF23+mice appeared the osteoporosis features, including the reduced length and volume of the
femur and mandible, the decrease in bone mineral density, the increase in light transmittance of X-ray in mandibular molar, incisor, and
alveolar, the widen pulp cavity widened, and the thinner root canals. 2) The FD values of femoral head and metaphyses significantly
increased in 1琢(OH)ase-/-, FGF23+ and 1琢(OH)ase-/-FGF23+ mice (P<0.05) while no significant differences were observed in femoral
meduallary cavity. Additionally, the FD value of the mandibular ramus significantly increased in 1alpha(OH)ase-/-, FGF23+ and 1alpha(OH)
ase-/-FGF23+mice (P<0.05) while no significant differences was observed in the alveolar bone. 3) The FD values in mandibular ramus was
significantly related to those in metaphysis (r=0.514, P=0.049).Conclusion:FD values could reflect the status of osteoporosis, the femoral
metaphysis and mandibular ramus were more suitable regions of interest. Moreover, the fractal analysis could be used in the evaluation of
the mandible imaging, and the optimized method might be expected to an auxiliary tool in the diagnosis of osteoporosis. |
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