| 宋珍 李镛 阴振晨 李擎天.抗菌肽人beta防御素3 对铜绿假单胞菌PAO-1 株的抑制作用[J].现代生物医学进展英文版,2014,14(32):6225-6228. |
| 抗菌肽人beta防御素3 对铜绿假单胞菌PAO-1 株的抑制作用 |
| The Inhibition on Pseudomonas Aeruginosa PAO-1 by AntimirobialPeptide Human beta Defensin 3 |
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| DOI： |
| 中文关键词: 抗菌肽 人beta防御素3 铜绿假单胞菌 抑菌试验 |
| 英文关键词: Antimicrobial peptide human beta defensin 3 Pseudomonas aeruginosa Antibacterial Test |
| 基金项目:国家自然科学基金项目（81000708） |
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| 中文摘要: |
| 目的：分析抗菌肽人beta防御素3 (human beta defensin 3，hBD3) 对铜绿假单胞菌PAO-1 株的抑制作用。方法：合成抗菌肽hBD3，分别通过最低抑菌浓度（minimal inhibitory concentration，MIC）检测、直接杀菌试验、重要功能基因检测分析其对PAO-1 的
直接抑制作用；并将其与阿奇霉素、四环素、利福平、氯霉素、链霉素、环丙沙星联合施用，观察对抗生素MIC 的影响。结果：HBD3对PAO-1 的MIC 为32 ug/mL；在浓度达到8 ug/mL时即有明显杀菌作用。HBD3 上调PAO-1 株的ahpF基因表达，下调aprA 和rhlR 基因表达。在联用5 ug/mL的hBD3后，四环素、利福平、氯霉素、链霉素、环丙沙星的MIC 值均有降低。结论：抗菌肽hBD3
对铜绿假单胞菌PAO-1 株有显著的抑制作用。 |
| 英文摘要: |
| Objective:To analyze the inhibition effects of antimicrobial peptide human beta defensin 3 (hBD3) against Pseudomonas
aeruginosa PAO-1.Methods:Antimicrobial peptide hBD3 was synthesized. The direct inhibition on PAO-1 was detected with
the MIC (minimal inhibitory concentration) test, antibacterial activity test and the analysis on some important functional genes. The peptide
was combined with azithromycin, tetracycline, rifampin, chloramphenicol, streptomycin, and ciprofloxacin for the effect on antibiotic
MIC values.Results:The MIC of hBD3 against PAO-1 is 32 ug/mL, the bactericidal activity was observed while the hBD3 concentration
as high as 8 ug/mL. The peptide hBD3 can improve the expression of ahpF and reduce the expression of aprA and rhlR. When 5 ug/mL
peptide was added, the MIC values against PAO1 of tetracycline, rifampicin, chloramphenicol, streptomycin were dropped down.Conclusion:There is considerable bactericidal activity of antimicrobial peptide hBD3 against P. aeruginosa PAO1. |
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