| 刘向东 艾子敏 张燕 马海军 张连云.慢性肾小球肾炎患者血清MMP-9 和TIMP-1 的浓度及意义[J].现代生物医学进展英文版,2014,14(31):6129-6134. |
| 慢性肾小球肾炎患者血清MMP-9 和TIMP-1 的浓度及意义 |
| Concentration and Significance of MMP-9 and TIMP-1 in Serumof Patientswith Chronic Glomerulonephritis |
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| DOI： |
| 中文关键词: 慢性肾炎 基质金属蛋白酶9 金属蛋白酶组织抑制因子1 细胞外基质 |
| 英文关键词: Chronic Glomerulonephritis MMP-9 TIMP-1 Extracellular matrix |
| 基金项目: |
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| 中文摘要: |
| 目的：观察慢性肾小球肾炎血清基质金属蛋白酶9 (matrix metalo protein- ase-9, MMP-9）、金属蛋白酶组织抑制剂1（tissue
inhibitors of metalloproteinases , TIMP-1）的浓度与肾组织中MMP-9、TIMP-1 表达的相关性，探讨慢性肾小球肾炎血清MMP-9、
TIMP-1 对肾脏纤维化的判断价值。方法：通过肾组织活检病理检查，将入选慢性肾炎的病例分增生组（A 组）15 例，纤维化组（B
组）15 例，另选10 例志愿者作为健康对照组C 组。应用免疫组化法观察A、B 两组MMP-9、TIMP-1在肾组织中的表达情况，并且
进行半定量分析，比较它们之间有无差别。应用ELISA 双抗体夹心法检测A、B、C 三组MMP-9、TIMP-1 在血清中的浓度，比较它
们之间有无差别。观察A、B两组MMP-9、TIMP-1在肾组织中的表达水平与在血清的浓度有无相关性。结果：A、B 两组MMP-9 在
肾小球和肾间质少见表达，主要在肾小管上皮细胞浆中表达增高，两组之间表达的强度有显著差异性；A组TIMP-1 在肾小球中
少见表达，在肾小管上皮细胞增强。B 组TIMP-1在肾小球中有少量表达，在肾小管上皮细胞较A 组进一步增强，两组之间表达的
强度有显著差异性（P<0.05）。血清中MMP-9、TIMP-1 浓度在A、B 组显著高于C 组，血清中MMP-9 在A、B 两组之间无显著差
异性，血清中TIMP-1 在A、B、C 三组间两两比较有显著差别（P<0.05）。结论：慢性肾炎患者血清中MMP-9、TIMP-1 浓度与肾脏
组织中MMP-9、TIMP-1 的表达呈正相关。MMP-9、TIMP-1 的相关性分析P值小于0.01。血清MMP-9、TIMP-1 参与了肾脏纤维化
的进展，慢性肾小球肾炎血清中MMP-9、TIMP-1 的浓度可在一定程度上反映肾脏纤维化程度。 |
| 英文摘要: |
| Objective:To explore the relationship between serum MMP-9 and TIMP-1 in serum and the mechanism of renal
fibrosis by means of detecting the concentrations and expressions of MMP-9 and TIMP-1 in serum of patients with chronic
glomerulonephritis.Methods:30 patients with chronic glomerulonephritis were divided into the proliferation group (group A, n=15) and
fibrosis group(group B, n=15) according to renal fibrosis degree in the renal pathology. And another 10 healthy people were chosen as the
control group (group C, n=10). The expressions of MMP-9 and TIMP-1 in the renal tissue were detected by the immunohistochemistry
and half quantitative analysis. The expressions of MMP-9 and TIMP-1 in the renal tissue between the two groups were compared. Then
the relationship between the concentrations of serumMMP-9 and TIMP-1 and the renal disease was analyzed.Results:The expression of
MMP-9 was low in the glomerulus and renal interstitial. The expression of MMP-9 was very high in tubular epithelial cells in group A
and group B. There was statistically significant differences between two groups (P<0.05). The expression of TIMP-1 was lower in the
glomerulus and higher in tubular epithelial cells in group A. The expression of TIMP-1 in tubular epithelial cells in group B was higher
than that of group A. There was statistically significant difference between the two groups (P<0.01). The concentration of serum MMP-9
and TIMP-1 was significantly higher than that in the control group (P<0.05; P<0.01). There was no statistically significant difference in
the concentrations of MMP-9 between group A and group B (P>0.05). The expression of TIMP-1 in the renal tissue has a positive
correlation with the level of TIMP-1 in serum of patients (r=0.489).Conclusion:The level of serum MMP9 and TIMP-1 were positively
correlated with the expressions in renal tissue. The concentration of MMP-9 and TIMP-1 in serum could reflect the renal fibrosis degree
of patients with the chronic glomerulonephritis. |
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