| 张奇昕 原明璐 陈顺 袁伟恩.新型可降解PEI 衍生物的表征及其在Brl-3A细胞中的毒性和转染研究[J].现代生物医学进展英文版,2014,14(31):6032-6034. |
| 新型可降解PEI 衍生物的表征及其在Brl-3A细胞中的毒性和转染研究 |
| Characterizations of Novel Degradable Polyethyleneimine Derivativesand Transfection and Cytotoxicity Studies on Brl-3A |
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| DOI： |
| 中文关键词: 基因载体 PEI衍生物 细胞毒性 转染效率 比例 |
| 英文关键词: General carrier PEI derivative Cytotoxicity Transfection Ratio |
| 基金项目:国家自然基金项目（81373366） |
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| 中文摘要: |
| 目的：对新型可降解高分子进行表征，研究其在Brl-3A 细胞中的毒性和转染效率，以及连接剂比例对以上方面的影响。方
法：通过化学方法合成不同比例PEI-Tr 高分子，考察其包裹质粒DNA 形成纳米颗粒的粒径和电位，以CCK-8 方法考察Brl-3A
细胞中的细胞毒性，以荧光素酶质粒为报告基因考察Brl-3A 细胞中的转染效率。结果：PEI-Tr 材料能形成200 nm以下带20 mV
左右正电荷的纳米颗粒，具有较好的细胞内吞能力和溶液稳定性，细胞毒性实验证明，随着浓度增加PEI-Tr 材料显示了远低于
PEI-25kDa 的细胞毒性，细胞转染实验表明其拥有高效输送质粒的能力。结论：PEI-Tr 是一种高效低毒的可降解聚阳离子载体，在
基因输送领域有很大的潜力；连接剂的比例在聚阳离子功能中起到重要作用。 |
| 英文摘要: |
| Objective:To characterize a novel biodegradable polymer, investigate its cell cytotoxicity and gene transfection
efficiency, and the influence of linker's ratio.Methods:PEI-Tr series were synthesized, and the particle size and Zeta potential of
polymer-DNA nanoparticles were estimated, then, cell cytotoxicity and transfection efficiency were tested.Results:PEI-Tr could form
nanoparticles under 200 nm, and its Zeta potential was about 20 mV, which indicated that it had a good cell endocytosis capability and
stability. The Brl-3A cytotoxicity and transfection results suggested that PEI-Tr had a high plasmid transfection ability within low cell
toxicity.Conclusion:PEI-Tr is a promising, efficient and low-toxic gene carrier, and the linker ratio is an important factor in carrier
design. |
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