| 韩雨晨 李菲菲 杨磊 李明伟 刘余聪 杨雪静 冯雪.牵张应力对成骨肉瘤细胞MG-63 中RhoA和ROCK表达的影响[J].现代生物医学进展英文版,2014,14(31):6011-6013. |
| 牵张应力对成骨肉瘤细胞MG-63 中RhoA和ROCK表达的影响 |
| Effects of Stretch Stress on RhoA and ROCK Expression in MG-63 Cells |
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| DOI： |
| 中文关键词: MG-63 RhoA ROCK 机械牵张应力 表达 |
| 英文关键词: MG-63 RhoA ROCK Mechanical stretch stress Expression |
| 基金项目:国家自然科学基金青年科学基金项目（31200706） |
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| 中文摘要: |
| 目的：探索机械牵张应力对人成骨肉瘤细胞MG-63 中RhoA/ ROCK信号通路的影响。方法：将同一条件培养的细胞MG-63
分为实验组(加力)与对照组(不加力)，实验组采用Flexcell 牵张应力加载系统，选择12 %形变率作为加载应力值，分为五个时间
组，分别加载1 h，4 h，8 h，12 h，24 h，RT-PCR 检测RhoA、ROCK mRNA水平表达的变化，Western-Blot 检测RhoA与Rock 蛋白
含量变化。结果：RT-PCR 显示MG-63 细胞受应力刺激后1 h RhoA、ROCK均未见明显变化(P>0.05)，4 小时后略有升高(P<0.05)，
在8 h达到最大值(P<0.05)，12、24 h降低，但仍高于对照组(P<0.05)；Western-Blot 显示MG-63 细胞受应力刺激后1 h RhoA、
ROCK 均未见明显变化，4 h仍未见明显变化，在8 h达到最大值，12、24 h降低，高于对照组。结论：机械牵张力加载下MG-63 细
胞内RhoA、ROCK 表达升高并随时间增加出现峰值，提示其可能在成骨细胞力学信号转导过程中发挥重要作用。 |
| 英文摘要: |
| Objective:To investigate the effects of mechanical strain on RhoA and ROCK expression in osteoblasts MG-63.Methods:Mechanical strain at 12%was applied to MG-63 cells for 0(control group), 1, 4, 8, 12 and 24 hours respectively in vitro. RhoA
and ROCK mRNA expression in the cells was examined by RT-PCR, RhoA and ROCK expressions were examined by Western Blot.Results:RT-PCR showed that mRNA expressions of RhoA and ROCK were not significantly changed after 1 h strain treatment(P>0.05)
and slightly increased within 4 hours of mechanical strain application. Then the expression rose to the highest value after 8 hours and
decreased after 12 and 24 hours (P<0.05). Western Blot represented a similar result. The protein expression was changed and up to the
top after 8 hours then decreased gradually after 12 and 24 hours (P<0.05).Conclusion:Mechanical strain elongation regulates RhoA and
ROCK expression of MG-63 cells and the expression was up to a maximumvalue with the increase of time．It was speculated that RhoA
and ROCK may play an important role in cell signal transduction of mechanical strain. |
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