| 孙军健 王朝阳 贾永鹏 包爱敏 郑林丰.miR-26a抑制转化生长因子beta诱导的韧带成纤维细胞增殖[J].现代生物医学进展英文版,2014,14(29):5655-5657. |
| miR-26a抑制转化生长因子beta诱导的韧带成纤维细胞增殖 |
| The Inhibition of miR-26a on Proliferation of Ligament Fibroblasts Inducedby Transforming Growth Factor-beta |
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| DOI： |
| 中文关键词: MiR-26a 成纤维细胞 转化生长因子-beta 细胞增殖 |
| 英文关键词: MiR-26a Fibroblasts TGF-beta Cell proliferation |
| 基金项目:国家留学基金资助，上海交通大学附属第一人民医院“优秀青年人才”基金资助 |
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| 中文摘要: |
| 目的：研究miR-26a 对转化生长因子-beta（Transforming growth factor-茁,TGF-beta)诱导的韧带成纤维细胞增殖的影响。方法：原代分离大鼠肩周韧带成纤维细胞，通过免疫荧光鉴定细胞纯度，应用脂质体细胞转染miRNA模拟物的方式过表达miR-26a，定量PCR 的方法检测miR-26a 模拟物的过表达效率，CCK8 法检测细胞增殖能力的变化，生物信息学预测的方法分析miR-26a 可能的
作用靶基因。结果：免疫荧光检测发现原代分离细胞中，Vimentin 阳性细胞在90 %以上；转染miR-26a 模拟物后，细胞内miR-26a水平显著高于阴性对照组（P<0.05）；TGF-beta刺激明显促进韧带成纤维细胞生长（P<0.01），而miR-26a 则对TGF-beta诱导的韧带成
纤维细胞增殖具有显著的抑制作用（P<0.01）；生物信息学预测显示SMAD1/4、EIF4G2、PTEN和MARK1可能是miR-26a 影响细
胞增殖的作用靶基因。结论：miR-26a 可抑制TGF-beta诱导的韧带成纤维细胞增殖。 |
| 英文摘要: |
| Objective:To investigate the effect of miR-26a on proliferation of Ligament fibroblasts induced by Transforming
growth factor-beta（TGF-beta).Methods:Primary culture ligament fibroblasts from ligaments of rat shoulder were identified by
immunofluorescence. The expression level of miR-26a was up-regulated by the mimic of miR-26a with liposomes, and determined the
efficiency by real-time PCR. The changes of cell proliferation were detected by CCK8 assay. The potential targeted-genes of miR-26a
were predicted by bioinformatics.Results:Vimentin immunofluorescence assay showed that the positive cells were above 90%. The
miR-26a level increased apparentlyafter the transfection with mimic (P<0.05). The proliferations of fibroblasts were markedly promoted
by TGF-beta(P<0.01), but miR-26a suppressed the cell proliferation (P<0.01). Bioinformatics analyze showed that SMAD1/4,EIF4G2,PTEN
and MARK1 were the potential targeted-genes of miR-26a to inhibit the fibroblasts proliferation.Conclusion:MiR-26a can
inhibit fibroblast proliferation which is induced by TGF-beta. |
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