高菊荣 张春光 晁洋 宋扬 王慧媛.丹皮酚对TNF-alpha诱导的人牙龈成纤维细胞黏附分子表达水平的影响[J].现代生物医学进展英文版,2014,14(28):5454-5457. |
丹皮酚对TNF-alpha诱导的人牙龈成纤维细胞黏附分子表达水平的影响 |
Influence of Paeonol on the Expression of Adhesion Moleculeson Human Gingival Fibroblasts |
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DOI: |
中文关键词: 人牙龈成纤维细胞 牙周炎 黏附分子 丹皮酚 |
英文关键词: Human gingival fibroblasts Periodontitis Adhesion molecules Paeonol |
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中文摘要: |
目的:研究丹皮酚(Paeonol,Pae)对TNF-alpha诱导的人牙龈成纤维细胞(human gingival fibroblasts,HGF)黏附分子表达水平的影响。方法:使用50 ng/mL 的肿瘤坏死因子(tumor necrosis factor,TNF)-alpha诱导刺激人牙龈成纤维细胞,后分别加入浓度为0、50、100、200 mmol/L的Pae 孵育培养2、4、6 天后,用Western blot 法检测细胞间黏附分子(intercellular adhesion molecule,ICAM)-1 以及血管细胞黏附分子(vascularcell adhesion molecule,VCAM)-1的蛋白表达水平,用逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测黏附分子的mRNA 表达水平。结果:TNF-alpha诱导的人牙龈成纤维细胞ICAM-1 和VCAM-1 的蛋白和mRNA 表达水平均显著高于空白对照组(P<0.05);不同浓度的丹皮酚可显著降低TNF-alpha诱导的人牙龈成纤维细胞ICAM-1 和VCAM-1 的蛋白和mRNA表达(P<0.05),且随Pae 浓度的升高逐渐减少。当Pae 浓度达到100 mmol/L,蛋白和mRNA表达水平的抑制效果达到稳定程度(P>0.05),当Pae 浓度达到200 mmol/L 时,ICAM-1 和VCAM-1 mRNA 抑制效果达到诱导前的水平(P>0.05)。诱导时间的长短对蛋白和mRNA 表达水平抑制效果没有显著影响(P>0.05)。结论:丹皮酚可显著降低TNF-alpha诱导的人牙龈成纤维细胞黏附分子表达水平。 |
英文摘要: |
Objective:To investigate the influence of Paeonol on human gingival fibroblasts (HGF) stimulated by adhesion
molecules.Methods:HGF were stimulated by 50 ng/mL tumor necrosis factor (TNF)-alpha, then different concentrations (0, 50, 100, 200
mmol/L) of Pae were added to the experimental group. The protein and mRNA expression of intercellular adhesion molecule (ICAM)-1
and vascular cell adhesion molecule (VCAM)-1 were examined by western blot and reverse transcription polymerase chain reaction
(RT-PCR).Results:The protein and mRNA expression of ICAM-1 and VCAM-1 were increased by TNF-alpha stimulation (P<0.05), while
Pae inhibited the expression of ICAM-1 and VCAM-1 with the increase of Pae concentration (P<0.05). There was no significant effect of
inhibition on the expression of the protein and mRNA levels in induction time (P>0.05).Conclusion:Paeonol had the potential to provide
new method for the treatment of periodontal disease. |
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