| 黎奔 麦葭沂 钟玲 廖康生 刘博 郭建文.LC-MS/MS法测定大鼠血浆中的野黄芩苷含量[J].现代生物医学进展英文版,2014,14(28):5414-5418. |
| LC-MS/MS法测定大鼠血浆中的野黄芩苷含量 |
| Determination of Scutellarin in Rat Plasma by LC-MS/MS |
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| DOI： |
| 中文关键词: LC-MS/MS 灯盏生脉胶囊 野黄芩苷 药代动力学 |
| 英文关键词: LC-MS/MS Dengzhanshengmai capsule Scutellarin Pharmacokinetics |
| 基金项目:广东省科技计划项目（2012B031800310） |
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| 中文摘要: |
| 目的：建立LC-MS/MS的分析方法测定大鼠血浆中的野黄芩苷，研究灯盏生脉胶囊中野黄芩苷在大鼠体内的药动学行为。
方法：以噻氯匹定为内标，血浆样品经1%甲酸乙腈沉淀蛋白处理后，用LC-MS/MS 法测定血浆中的野黄芩苷浓度。结果：野黄芩
苷线性范围为1.31～670.00 ng·mL-1( γ>0.999)，最低定量浓度为1.31 ng·mL-1，回收率、日内、日间考察均符合生物样品分析要求。
实验结果显示，野黄芩苷在大鼠体内出现多峰现象。结论：建立的LC-MS/MS 定量分析方法灵敏、准确，可用于大鼠血浆中野黄芩
苷的测定及其药代动力学研究。 |
| 英文摘要: |
| Objective:To develop a new LC-MS/MS method for determination of scutellarin of Dengzhanshengmai capsule in rat
plasma. That method was applied to the pharmacokinetic study of scutellarin in rat.Methods:Ticlopidine was used as internal standard,
plasma samples were determined by LC-MS/MS with acetonitrile protein precipitation.Results:The calibration curves were linear in the
concentration range of 1.31～670.00 ng·mL-1 and the correlation coefficient was better than 0.999. The lower limit of quantitation was
1.31 ng·mL-1. The recovery rate, within-day and between-day variances met the requirement of biological sample analysis. The experiment
results showed that the drug concentration-time curve presented multi-peak phenomenon in rats.Conclusion:A sensitive and accurate
method was successfully applied to the pharmacokinetic study of scutellarin in rat plasma. |
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