| 刘海波 王慧慧 郑育红 王鼎 孙筱放.229 例流产绒毛染色体核型分析及两种长期培养方法比较[J].现代生物医学进展英文版,2014,14(28):5410-5413. |
| 229 例流产绒毛染色体核型分析及两种长期培养方法比较 |
| Cytogenetic Study on the Chorionic Villi in 229 Casesof Early Spontaneous Abortus and Comparationof two Long-termCulture Methods |
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| DOI： |
| 中文关键词: 自然流产 绒毛 染色体核型分析 细胞培养 |
| 英文关键词: Spontaneous abortion Chorionic villus Karyotype analysis Cell culture |
| 基金项目:国家自然科学基金项目(81302079)；广州医科大学青年基金项目(2012C10)；
广州医科大学附属第三医院青年基金项目(2012Y15) |
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| 中文摘要: |
| 目的：探讨早期自然流产绒毛染色体核型分析在自然流产病因检测中的应用价值，并比较两种长期培养方法的差别。方法：
选择孕早期自然流产的孕妇229 例，在无菌条件下，从宫腔内取出绒毛，同时或单独经胰酶消化法与切碎贴壁法进行细胞培养，
传代之后常规进行G 显带，在显微镜下做核型分析。结果：229 例流产胎儿绒毛，培养成功206 例，成功率为89.96％。异常核型
105例，异常率为50.97%，数目异常者101 例，占异常核型的96.19%，以16 三体最为多见。胰酶消化法的培养成功率及收获时间
都显著优于直接贴壁法，差异具有统计学意义(P<0.05)。结论：自然流产绒毛染色体核型分析对流产查因具有实用价值。胰酶消化
法较切碎贴壁法对流产绒毛长期培养及染色体核型分析更具实用性。 |
| 英文摘要: |
| Objective:To investigate the application of karyotypes of chorionic villi in the first trimester abortus and compare
trypsin digestion method with minced adherence method in the isolation and culture of chorionic villi samples in spontaneous abortions.Methods:A total of 229 cases of abortus were collected in sterile condition. Trypsin digestion method and minced adherence method
were used respectively in chorionic villi cells isolation and the culture of these abortus. These chorionic villi cells were subcultrued. Their
karyotypes were analysed by G band.Results:There were 105 cases with chromosomal abnormalities in the 206 successful cultured
abortus over all 299 cases. There were 101 cases with chromosomal numberical abnormality, accounting for 96.19% of chromosomal
abnormality. Trisomy 16 was the most common abnormality. Trypsin digestion method had higher success rate and shorter harvest time
than minced adherence method (P<0.05).Conclusion:Karyotype analysis of abortus villi was benefit to identify the abortion reason and
could guide the next pregnancy. Trypsin digestion method used in cytogenetic study of chorionic villi samples in spontaneous abortions
was more practicable and efficient. |
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