| 李宁 赵树立 徐贞俊 翁磊华 侯亚义.灵芝多糖对单核巨噬白血病细胞株的体外作用[J].现代生物医学进展英文版,2014,14(27):5238-5241. |
| 灵芝多糖对单核巨噬白血病细胞株的体外作用 |
| The Dual Effects of Ganoderma LucidumPolysaccharides onMonocyte-Macrophage Leukemia Cell Lines In Vitro |
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| DOI： |
| 中文关键词: 灵芝多糖 单核巨噬白血病细胞 死亡受体信号通路 细胞因子 |
| 英文关键词: Ganoderma lucidum polysaccharide (GLP) Monocyte-macrophage leukemia cell Death receptor signaling pathway Cytokines |
| 基金项目:济南大学博士基金（XBS1346） |
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| 中文摘要: |
| 目的：研究灵芝多糖对单核巨噬白血病细胞THP-1和RAW264.7 的肿瘤生物学活性的影响。方法：用1、50 和100 μg/ml的
灵芝多糖和脂多糖（LPS）刺激THP-1 和RAW264.7 细胞，CCK-8 法测定巨噬细胞的增殖活力、流式细胞仪检测细胞的凋亡活性、Q-PCR检测细胞因子TNF-α、IL-1、IL-6 和IL-12 基因、相关凋亡信号通路基因TRADD、TNFSF10、TNFRSR10b、NFκBI、Caspase10 和Caspase 3基因的mRNA 表达水平。结果：灵芝多糖可以呈反浓度依赖性地刺激两种细胞的增殖，在50 和100 μg/ml 浓度下
可引起细胞凋亡，凋亡信号基因TNFRSR10b 和NFκBI的mRNA 水平在24h 升高了53%和48%；在1~100 μg/ml 浓度下可上调细胞因子TNF-α、IL-1、IL-6和IL-12 细胞因子mRNA 的表达水平。结论：灵芝多糖对白血病细胞株具有双重作用，在低浓度下促
进细胞增殖，而在高剂量时诱导细胞凋亡，均可上调免疫相关细胞因子的表达。 |
| 英文摘要: |
| Objective:To study the effects of Ganoderma lucidum polysaccharides (GLP) on tumor biology of the monocyte-
macrophage THP-1 and RAW264.7.Methods:After THP-1 and Raw264.7 cells were stimulated with different concentrations of
GLP and LPS, the proliferation and apoptosis activity of cells were analyzed by CCK-8 kits and flow cytometry. RT-PCR was used to detect
expression of cytokines of macrophages,such as TNF-α, IL-1, IL-6 and IL-12, and Q-PCR was used to detect the gene expression
levels in apoptosis signaling pathway.Results:GLP could promote proliferation of THP-1 and RAW264.7 cells in an inverse dose-dependent
, and up-regulate expression of cytokines secretion at all concentrations. And also GLP could elevate expression of apoptosis related
genes TNFRSR10b and NFκBI.Conclusion:GLP played a dual role on leukemia cell line, that is it increased expression of cytokines and
promoted cells proliferation at lower concentration, while at high doses caused cells apoptosis. |
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