柳天雄 罗佳 黄菊芳 曾乐平.古DNA提取技术新进展[J].现代生物医学进展英文版,2014,14(26):5170-5175. |
古DNA提取技术新进展 |
Progress in Ancient DNA Extraction Technology |
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DOI: |
中文关键词: 古DNA 古DNA提取技术 mtDNA 提取 |
英文关键词: Ancient DNA Ancient DNA extraction technology MtDNA extraction |
基金项目:国家“十二五”科技支撑计划课题资助项目(2012BAK14B03);
湖南省自然基金项目(12JJ4023) |
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中文摘要: |
古DNA 是揭示古代生物生长状态以及生物千百万年来进化情况的最重要的信息载体,在治疗人类遗传性的疑难病症及牲
畜饲养和粮食作物种植等方面都有重大的贡献。古DNA提取技术作为获得该重要的信息载体的最重要手段,长久以来受到了世
界各地的考古学家以及医学研究学者们的高度重视。随着科学的发展,古DNA提取技术已经形成多种核心方法:Chelex-100 法、
酚-氯仿抽提法、二氧化硅(硅粒)法、NaOH 法、硅离心柱法试剂盒、磁珠法试剂盒等方法。本文将根据最新的研究成果对以上提
到的几种方法进行分析比较,以期能够为将来古DNA提取技术的发展创新提供新的思路与方向。 |
英文摘要: |
Ancient DNA is the information carrier that reveal the ancient organism growing state and the evolutionary status of
living creature in millions of years. It brings the great contribution in human hereditary diseases treatment, crop cultivation and so many
other filed. Ancient DNA extraction technology, as the most important way to take the information carrier, has attracted the attention of
many scientists. With the development of scientific, ancient DNA extraction technology has formed of some conventional techniques:
Chelex-100 method, phenol and chloroform method and so on. Based on the latest research results, here we compared and analyzed the
above mentioned techniques so as to provide new ideas for the development of ancient DNA extraction technology. |
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