杨蕙宇 潘瑾 李平 唐蕊 向萌.Ets-1 在姜黄素抑制心脏纤维化的表达研究[J].现代生物医学进展英文版,2014,14(26):5045-5048. |
Ets-1 在姜黄素抑制心脏纤维化的表达研究 |
Curcumin Through Reduced Ets-1 Expression Induced Inhibition of AngIIHeart into Fibroblast Proliferation |
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DOI: |
中文关键词: Ets-1 姜黄素 血管紧张素-II 纤维化 |
英文关键词: Ets-1 Curcumin Angiotensin II Cardiac fibroblast |
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中文摘要: |
目的:研究姜黄素的抗增殖作用是否依赖于其对Ets-1 表达的下调。方法:使用Ets-1 siRNA 对CFs 细胞Ets-1 基因进行沉
默;Real-time PCR和western-blot 法测定各组细胞Ets-1 mRNA和蛋白的表达水平。结果:在mRNA和蛋白水平上,AngII明显
增加CFs 细胞内Ets-1 的表达;使用siRNA 技术对Ets-1 进行沉默后,随着Ets-1 表达的降低及转录调节能力的下降,由AngII 诱
导的CFs 细胞的增殖能力及增殖相关细胞因子分泌减少;姜黄素可以显著降低AngII 诱导的Ets-1 表达升高,并具有浓度依赖性
(P<0.05)。说明姜黄素对AngII诱导的CFs 增殖的抑制作用可能依赖于其对Ets-1 表达的抑制作用。结论:Ets-1 siRNA 对Ets-1 进
行基因沉默后,显示出抗增殖效用;姜黄素能够有效地抑制AngII诱导的CFs 细胞Ets-1 mRNA和蛋白的过表达;姜黄素的抗增殖
作用可能依赖于其对Ets-1 基因的表达下调而得以实现的。 |
英文摘要: |
Objective:To observe the inhibition effect of curcumin (Cur) on AngII-induced over-proliferation and overexpression
of CTGF and PAI-1 in CFs and relation between its inhibitory effect and the suppressing expression of Ets-1.Methods:Ets-1 were
measured by real-time PCR and western-blotting, respectively. After transfected with Ets-1 si-RNA, the cells were incubated with AngII
(100 nmol/L) for indicated times. The cell activity was detected by MTT assay and the levels of mRNA and protein of CTGF, PAI-1,
Ets-1 were measured by real-time PCR and western-blotting, respectively.Results:With the lower expression of Ets-1, the proliferation
which were induced by AngII was decreased in CFs cells and the expression of CTGF and PAI-1 also declined. Curcumin can
significantly reduced the expression of Ets-1 and the reduction was also concentration-related (P<0.05)Conclusion:Curcumin
significantly inhibits AngII-induced overexpression of Ets-1 in the levels of mRNA and protein in CFs, and Ets-1 siRNA also has the
same inhibitory effect as Cur. These results suggested that Cur's anti-fibrotic effect, in part, depends on its inhibitory effect on the
overexpression of Ets-1 in AngII-stimulated CFs. |
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