| 李甜 张亚楼 陈龙 秦纹 钟近洁.链脲佐菌素诱导C57小鼠1型糖尿病模型的研究[J].现代生物医学进展英文版,2014,14(26):5031-5033. |
| 链脲佐菌素诱导C57小鼠1型糖尿病模型的研究 |
| StudyofType1DiabetesModel InducedbyStreptozotocinintheC57Mouse |
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| DOI： |
| 中文关键词: 链脲佐菌素 1 型糖尿病 C57 小鼠 |
| 英文关键词: Streptozotocin Type 1 diabetes C57 mouse |
| 基金项目:新疆医科大学校内支撑学科项目-人体解剖与组织胚胎学阶段性成果（XYDXK20780307）；
国家自然科学基金项目(81160331，81360409)；新疆医科大学科研创新基金（XJC201134，XJC201229） |
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| 中文摘要: |
| 目的：通过小剂量多次腹腔注射链脲佐菌素（STZ）诱导建立与人类1 型糖尿病相似的C57 小鼠糖尿病模型，研究建模剂量
和成模率。方法：将32 只C57 小鼠随机分为正常对照组（A）和实验组（B）。实验组（B）可分为低、中、高剂量组（50 mg/kg、70
mg/kg、90 mg/kg）(n=8)。两组都喂普通饲料1 周后，B 组连续5 天腹腔注射不同剂量STZ，测定注射前、注射后1 周、2 周、3 周、4
周、5 周的空腹血糖和体重，观察小鼠饮食、饮水和排尿情况。STZ注射第3 周进行口服糖耐量实验（OGTT）。结果：给药前A、B 组
体重和血糖无显著差异，给药1 周后，B 组饮水量和进食量明显增加，体重减轻。C57小鼠用药2周后，中剂量组达到建模标准，成
模率75 %。各剂量组均出现了糖耐量异常。结论：诱导建立C57 小鼠1 型糖尿病模型方法是连续5 日腹腔注注射STZ，适宜剂量
为70 mg/kg。 |
| 英文摘要: |
| Objective:To establish a C57 mouse model of type 1 diabetes by multiple intraperitoneal injection of low
concentration STZ, and evaluate model effect and the success rate.Methods:32 C57 mice were randomly divided into normal control
group (A) and experimental group (B). The experimental group (B) was divided into low, middle, high dose group (50 mg/kg, 70 mg/kg,
90 mg/kg) (n=8). The two groups were fed with normal diet for 1 week. Group B received different concentration of STZ for 5
consecutive days by intraperitoneal injection. Blood glucose were determined at pre-injection, 1 weeks after injection, 2 weeks, 3 weeks,
4 weeks, 5 weeks and body weight were also detected. At the same time animal's eating, drinking and voiding were recorded. 3 weeks
after STZ injection. Oral glucose tolerance test (OGTT) was carried out at the same time.Results:Body weight and blood glucose group
A and group B had no significant difference between group A and group B before drug administration. After treatment for 1 week, mice
in group B had water intake and food intake increased, but weight lost. After 2 weeks medication, the C57 mice in middle dose group
reached the standard of modeling, and the successful rate was 75%. Each dose group showed an impaired glucose tolerance.Conclusion:The establishment of type 1 diabetes model of C57 mice can be induced by STZ, with consecutive 5 days intraperitoneal injections, and
suitable dose of 70 mg/kg. |
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