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目的：研究DEC1 在肝癌（hepatocellular carcinoma，HCC）患者血清中的表达水平及其临床意义。方法：收集60 例HCC 患者，30 例正常人，30 例慢性肝炎患者，30例慢性肝硬化患者血清标本。采用实时荧光定量RT-PCR (RT-PCR)检测血清DEC1 的表达情况。分析血清DEC1 的表达水平与AFP相关性。探讨血清DEC1 的表达水平与HCC手术预后的关系。结果：肝癌患者血清 DEC1 明显高于正常人、慢性肝炎和慢性肝硬化患者（P<0.05）。皮尔森相关分析结果显示HCC患者血清DEC1的表达与血清 AFP 水平是呈正相关的。Kaplan-Meier 结果显示血清DEC1 低表达组术后的复发转移率显著低于高表达组，其术后生存率也显著高于高表达组（P<0.01）。结论：DEC1在肝癌者血清中高表达，不仅可能成为HCC 诊断的标记物，且其血清表达水平也有助于 HCC 临床预后的评估。

英文摘要:

Objective:To investigate the expression of DEC1 in serum of patients with the hepatocellular carcinoma (HCC), as well as their clinical significances.Methods:The blood samples were collected from 60 patients with HCC, 30 patients with chronic hepatitis, 30 patients with chronic hepatic cirrhosis and 30 normal subjects. The reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of DEC1 in the serum. Pearson's correlation analysis was used to estimate the relationship between DEC1 and AFP. The correlations of DEC1 expression to the prognosis were analyzed.Results:The expression of DEC1 level in the serum of patients with HCC was significantly higher than those of the benign diseases and the healthy controls(P< 0.05). Pearson's correlation analysis showed that DEC1 expression level in serumhad a close correlation with AFP (P<0.05). The survival time of HCC patients with a lower DEC1 expression level was longer than those with a higher 1eve1, and the recurrent/metastatic of HCC patients with a lower DEC1 expression level was also lower than those with higher expression (P<0.05).Conclusion:The expression of DEC1 in serummay serve as a promising and prognostic biomarker for HCC.

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