刘甜 苏映军 陈晨 陈建武 刘蓓 杨阳 张栋梁 郭树忠.诱导性调节性T 细胞抑制移植排斥反应的作用研究[J].现代生物医学进展英文版,2014,14(25):4826-4831. |
诱导性调节性T 细胞抑制移植排斥反应的作用研究 |
Suppressive Function of Induced Regulatory T Cells in Allograft Rejection |
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DOI: |
中文关键词: 调节性T 细胞 细胞培养 移植排斥反应 |
英文关键词: Regulatory T cell Cell Culture Allograft Rejection |
基金项目:国家自然科学基金重点项目(30830102);国家自然科学基金面上项目(81071581) |
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中文摘要: |
目的:通过体外诱导的方法将幼稚CD4+ T细胞(naive CD4+T cell)转化为调节性T 细胞(Regulatory T cells,Tregs),并验证
其在小鼠异体皮片移植模型上对移植排斥反应的抑制作用。方法:分选na觙ve CD4+ T 细胞并在体外诱导其转化为Tregs,流式检测
细胞确定其转化率。将诱导性Treg(induced Treg,iTreg)与效应T细胞(Effective T cells,Teffs)以不同比例共同培养检测其对T 细
胞增殖的抑制能力。建立C57bl/6 到Balb/c 小鼠的异体皮片移植模型,植皮术后将iTreg 经由股静脉输注入受体(Balb/c 小鼠)体
内,观察皮片存活情况,绘制皮片存活曲线。同时于皮片移植术后11 天对皮片进行病理切片,观察移植排斥反应状况。结果:体外
诱导naive CD4+T 细胞转化为iTreg 的比例约44%,在iTreg:Teff比例大于1:4 时,iTreg 具有明显地抑制Teff增殖的作用,且这
种抑制作用具有剂量依赖性。植皮小鼠输注iTreg 后皮片存活时间较对照组延长约2.4 天,病理切片显示排斥反应减轻,但皮片在
14 天左右时仍被排斥。结论:体外诱导的iTreg能够在体外抑制Teff增殖,且能有效抑制小鼠异体皮片移植后排斥反应。 |
英文摘要: |
Objective:Induced regulatory T cells (iTregs) have been implicated in maintaining peripheral immune tolerance. This
study aims to test whether iTregs could suppress allograft rejection in a skin allograft model.Methods:Na觙ve CD4+T cells were purified
from na 觙ve mice splenocytes and induced in vitro by culturing with anti-CD3/CD28-coated Dynalbeads, and supplemented with
interleukin(IL)-2 and transforming growth fator(TGFbeta). Suppressive ability of iTregs was assayed by co-culturing with CD4+CD25-T cells
(Teffs) in vitro and by allogeneic skin transplantation in vivo.Results:Flow cytometry showed the percentage of CD4+CD25 + Foxp3+
cells in induced cells were approximately 44%. iTregs exerted suppressive function when cocultured with Teffs in vitro and prolonged
fully MHC-mismatched skin graft survival.Conclusion:iTreg cells show suppressive function both in vitro and in vivo, but iTreg cells
alone can not induce immune tolerance. |
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