| 刘甜 苏映军 陈晨 陈建武 刘蓓 杨阳 张栋梁 郭树忠.诱导性调节性T 细胞抑制移植排斥反应的作用研究[J].现代生物医学进展英文版,2014,14(25):4826-4831. |
| 诱导性调节性T 细胞抑制移植排斥反应的作用研究 |
| Suppressive Function of Induced Regulatory T Cells in Allograft Rejection |
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| DOI： |
| 中文关键词: 调节性T 细胞 细胞培养 移植排斥反应 |
| 英文关键词: Regulatory T cell Cell Culture Allograft Rejection |
| 基金项目:国家自然科学基金重点项目（30830102）；国家自然科学基金面上项目（81071581） |
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| 中文摘要: |
| 目的：通过体外诱导的方法将幼稚CD4+ T细胞（naive CD4+T cell）转化为调节性T 细胞（Regulatory T cells，Tregs），并验证
其在小鼠异体皮片移植模型上对移植排斥反应的抑制作用。方法：分选na觙ve CD4+ T 细胞并在体外诱导其转化为Tregs，流式检测
细胞确定其转化率。将诱导性Treg（induced Treg，iTreg）与效应T细胞（Effective T cells，Teffs）以不同比例共同培养检测其对T 细
胞增殖的抑制能力。建立C57bl/6 到Balb/c 小鼠的异体皮片移植模型，植皮术后将iTreg 经由股静脉输注入受体（Balb/c 小鼠）体
内，观察皮片存活情况，绘制皮片存活曲线。同时于皮片移植术后11 天对皮片进行病理切片，观察移植排斥反应状况。结果：体外
诱导naive CD4+T 细胞转化为iTreg 的比例约44％，在iTreg：Teff比例大于1：4 时，iTreg 具有明显地抑制Teff增殖的作用，且这
种抑制作用具有剂量依赖性。植皮小鼠输注iTreg 后皮片存活时间较对照组延长约2.4 天，病理切片显示排斥反应减轻，但皮片在
14 天左右时仍被排斥。结论：体外诱导的iTreg能够在体外抑制Teff增殖，且能有效抑制小鼠异体皮片移植后排斥反应。 |
| 英文摘要: |
| Objective:Induced regulatory T cells (iTregs) have been implicated in maintaining peripheral immune tolerance. This
study aims to test whether iTregs could suppress allograft rejection in a skin allograft model.Methods:Na觙ve CD4+T cells were purified
from na 觙ve mice splenocytes and induced in vitro by culturing with anti-CD3/CD28-coated Dynalbeads, and supplemented with
interleukin(IL)-2 and transforming growth fator(TGFbeta). Suppressive ability of iTregs was assayed by co-culturing with CD4+CD25-T cells
(Teffs) in vitro and by allogeneic skin transplantation in vivo.Results:Flow cytometry showed the percentage of CD4+CD25 + Foxp3+
cells in induced cells were approximately 44%. iTregs exerted suppressive function when cocultured with Teffs in vitro and prolonged
fully MHC-mismatched skin graft survival.Conclusion:iTreg cells show suppressive function both in vitro and in vivo, but iTreg cells
alone can not induce immune tolerance. |
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