| 暴蕾 段海霞 王则绯.蟾毒灵对人卵巢癌SKOV-3 细胞增殖的影响[J].现代生物医学进展英文版,2014,14(24):4662-4665. |
| 蟾毒灵对人卵巢癌SKOV-3 细胞增殖的影响 |
| Effect of Bufalin on Proliferation of Human Ovarian SKOV-3 Cells |
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| DOI： |
| 中文关键词: 蟾毒灵 人卵巢癌SKOV-3 细胞 细胞凋亡 |
| 英文关键词: Bufalin Human ovarian SKOV-3 cells Apoptosis |
| 基金项目:陕西省自然科学基金项目（2013K12-03-22） |
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| 中文摘要: |
| 目的：探讨蟾毒灵对人卵巢癌SKOV-3细胞增殖抑制和凋亡的影响，为卵巢癌临床治疗提供依据和分子基础。方法：不同浓
度蟾毒灵处理卵巢癌SKOV-3 细胞后，MTT 法检测细胞增殖抑制作用，细胞免疫化学染色法检测细胞的凋亡，Western Blot 法检
测Bax，Bcl-2，Caspase-3 蛋白以及计算Bax/Bcl-2 的比值。结果：蟾毒灵能够抑制SKOV-3 细胞的增殖，且成时间和剂量依赖性，
免疫荧光显示蟾毒灵对SKOV-3 细胞具有凋亡作用，Western Blot 检测发现蟾毒灵能够促进Caspase-3 蛋白的活化，提高
Bax/Bcl-2 的比值。结论：蟾毒灵在体外能够抑制卵巢癌SKOV-3 细胞的增殖和促进卵巢癌SKOV-3 细胞的凋亡。 |
| 英文摘要: |
| Objective:To study the inhibitory effect of bufalin on the proliferation and apoptosis of human ovarian cancer
SKOV-3 cells.Methods:human ovarian SKOV-3 cells were treated with diferent doses of bufalin. The inhibitory effect of bufalin on the
proliferation was detected by MTT assay. The cell apoptosis was determined by immunocytochemistry. Western Blot detected bufalin can
promote activation of Caspase 3 protein and the ratio of Bax/Bcl2.Results:Bufalin inhibited the proliferation of human ovarian SKOV-3
cells in a time-and dose-dependent manner．Bufalin induced apoptosis of human ovarian SKOV-3 cells. Bufalin promote activation of
Caspase 3 protein and the ratio of Bax/Bcl2.Conclusion:Bufalin inhibits the growth of ovarian cancer SKOV-3 cells in vitro and
promotes optosis through activation of Caspase 3 protein. |
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