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目的：探讨联合应用局部亚低温(32-35 ℃)及硫酸镁对局灶性脑缺血大鼠的保护作用及其可能机制。方法：通过线栓法建立大鼠大脑中动脉阻塞(MCAO)模型，将40 只雄性Wistar 大鼠随机分为假手术组、常温组、亚低温组、硫酸镁组、亚低温+ 硫酸镁组，每组8 例，采用Longa 神经功能评分、TTC 染色、TUNEL 技术，检测和比较各组脑缺血后大鼠的神经功能、脑梗死体积、凋亡细胞数。结果：与常温组相比，亚低温组与亚低温+硫酸镁组的梗死体积、神经功能评分、凋亡细胞数均明显降低，差异有显著意义(P<0.05)；而与亚低温组相比，亚低温+硫酸镁组局灶脑缺血大鼠的脑梗死体积、神经功能评分、凋亡细胞数均显著减少，差异有显著意义(P<0.05)。结论：与单独应用亚低温相比，局部亚低温与硫酸镁联合应用，对局灶性脑缺血大鼠可发挥更有效的脑保护作用，其机制可能与抑制脑缺血后凋亡有关。

英文摘要:

Objective:To investigate the protective effect of focal mild hypothermia combined with MgSO4 on the rats with focal cerebral ischemia and its possible mechanism.Methods:The middle cerebral artery occlusion(MCAO) models of rat were achieved by an intraluminal filament. Forty male adult Wistar rats were randomly divided into sham-operated group, normothermia ischemia group, MgSO4 group, mild hypothermia group and mild hypothermia+ MgSO4 group (each group n=8). The neurological function, infarct volumes, apoptotic cell numbers in the ischemic cortex were assessed by Longa neurological scores, TTC staining and TUNEL.Results:Compared with the normothermia group, the neurological scores, infarct volumes and apoptotic cell numbers markedly decreased in hypothermia group and hypothermia+MgSO4 group (P<0.05). Compared with mild hypothermia group, the neurological scores, infarct volumes and apoptotic cell numbers markedly decreased in hypothermia+MgSO4 group (P<0.05).Conclusion:Focal mild hypothermia combined with MgSO4 was more effective in the protection of rats with focal cerebral ischemia than mild hypothermia, which was partly due to the decrease of apoptosis.

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