| 高宇 赵三军 王培欢 芦帅 李晓静 陈吉华.蛋白聚糖在维持牙本质胶原空间形貌和水合性能方面的作用[J].现代生物医学进展英文版,2014,14(21):4001-4004. |
| 蛋白聚糖在维持牙本质胶原空间形貌和水合性能方面的作用 |
| Effect of Proteoglycans on the Maintain Collagen Morphology and HydrationStatus of Human Dentin |
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| DOI： |
| 中文关键词: 胶原纤维 牙本质 糖胺聚糖侧链 蛋白聚糖 |
| 英文关键词: Collagen Dentin Glycosaminoglycans Proteoglycans |
| 基金项目:国家自然科学基金项目(81130078；81170985) |
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| 中文摘要: |
| 目的：评价牙本质蛋白聚糖对脱矿牙本质胶原纤维形貌和水合性能的影响。方法：新鲜拔除无龋坏人磨牙牙本质酸蚀后分
别用胰蛋白酶和硫酸软骨素酶ABC孵育去除牙本质蛋白聚糖和糖胺聚糖侧链，对照组与实验组处理方法相同，但孵育液中不添
加酶。然后在牙本质表面不同润湿状态下用场发射扫描电镜和激光共聚焦扫描电镜分别观察牙本质的微观形貌并评价脱矿牙本
质的水合性能。结果：硫酸软骨素酶ABC 和TRY酶处理改变了牙本质的微观形貌，使胶原纤维间距增大。酶处理、牙本质表面润
湿性及两者的交互作用均会显著影响脱矿牙本质的厚度(P<0.0001)。结论：牙本质蛋白聚糖和糖胺聚糖侧链在维持牙本质胶原纤
维网的空间结构和水合作用方面均发挥着重要作用。蛋白聚糖、胶原纤维- 蛋白聚糖以及蛋白聚糖-蛋白聚糖间的的亲水性是影
响脱矿牙本质围观形貌和厚度的重要因素。 |
| 英文摘要: |
| Objective:To evaluate the effect of proteoglycans on the maintain collagen morphology and hydration status of human
dentin.Methods:Caries-free, human third molar dentin was acid-etched and treated with trypsin or chondroitinase ABC to remove the
proteoglycans and attached glycosaminoglycans. Controls were prepared in the same manner but without using the enzymes. After
digestion, FESEM and CLSM were used to evaluate the collagen morphology and hydration effects of dentin, respectively.Results:Digestion with trypsin or chondroitinase ABC induced enlarged inter fibrillar spaces of acid-etched dentin and altered the collagen
morphology. The factor enzyme treatment (P<0.0001) and the factor surface wetness status (P<0.0001) both significantly affected the
thickness of acid-etching dentin. There was also significant difference for the factor interactions (P<0.0001).Conclusion:Glycosaminoglycans chains and proteoglycans were important in maintaining the spatial structure and hydration status of collagen fibril
scaffold. The hydrophilic nature of proteoglycans and the collagen- proteoglycans and proteoglycans- proteoglycans networks affected
the morphology and thickness of acid-etched dentin. |
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