冯艳 姜日文 于龙 华泽权.白介素-17 在牙龈上皮细胞中调控趋化因子的机制研究[J].现代生物医学进展英文版,2014,14(18):3438-3440. |
白介素-17 在牙龈上皮细胞中调控趋化因子的机制研究 |
Effect of Interleukin-17 on the Expression of Chemokines inGingivalepithelial Cells |
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DOI: |
中文关键词: 白介素-17 白介素-8 单核细胞趋化蛋白-1 NF-κB |
英文关键词: IL-17 CXCL8 CCL2 NF-κB |
基金项目:辽宁省科技攻关项目(201207223) |
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中文摘要: |
目的:有研究发现白介素-17(IL-17)作为炎症反应的重要标志物在炎症反应中具有重要作用,并且其表达水平的高低与牙
周疾病的严重程度有着正相关性,但是目前为止白介素-17 对于牙龈上皮细胞的趋化因子生成的影响尚没有研究,所以本课题主
要探索在牙龈上皮细胞(human gingival epithelial cells,HGECs)中白介素-17 如何通过趋化因子调控炎症反应,并进一步探究其
作用机制。方法:酶联免疫吸附剂测定法(enzyme linked immunosorbent assay,ELISA)观察细胞中相关趋化因子分泌,同时蛋白印
迹法(western blot)观察细胞中核转录因子κB的变化。结果:无IL-17 刺激组与IL-17 刺激组比较,IL-17 刺激组的趋化因子白介
素8(CXCL8)分泌量显著性增加而另一趋化因子单核细胞趋化蛋白-1(CCL2)却没有显著变化,并且通过磷酸化I资B 的表达量显
著性增加提示NF-κB 被激活(P<0.05);同时IL-17 刺激组与IL-17+NF-κB抑制剂组比较,IL-17 +NF-κB抑制剂组的CXCL8 分泌
量显著降低,而通过磷酸化I资B的表达量显著减少提示NF-κB 的活性被抑制(P<0.05)。结论:NF-κB 对IL-17 刺激下的牙龈上皮
细胞趋化因子的表达调控具有关键性作用,同时可能也为将来治疗IL-17 诱导的牙周炎症性疾病提供了新的靶点。 |
英文摘要: |
Objective:The role of interleukin (IL)-17 in cellular communication in inflammation has been well described, and a
positive correlation between the severity of periodontitis and the level of IL-17 was reported. Although epithelial cells are a major target
of IL-17, little is known about the effect of IL-17 on the production of chemokines by human gingival epithelial cells (HGECs). The
effects and the mechanism of IL-17 have been evaluated on the expression of CXCL8 and CCL2 by HGECs.Methods:After IL-17
stimulated the human gingival epithelial cells, We evaluated the effects of IL-17 on the expression of CXCL8 and CCL2 by HGECs using
ELISA. In addition, the role of the nuclear factor (NF)-κB signalling pathway in the IL-17-mediated expression of chemokines was
assessed using a specific inhibitor by WESTERN BOLT.Results:Comparing no stimulation of IL-17 group with under stimulation of
IL-17 group, the secretion of CXCL8 protein had significantly increased. And the protein expression of p-IκB significantly increased
indicate the activation of NF-κB (P<0.05). Meanwhile, comparing stimulation of IL-17 group with stimulation of IL-17 plus the inhibitor
of NF-κB group, the secretion of CXCL8 protein had significantly dcressed. And the protein expression of p-IκB significantly decressed
indicate the inhibition of NF-κB (P<0.05).Conclusion:IL-17 is involved in the regulation of the innate immune response in HGECs by
inducing CXCL8 production and the NF-jB signalling pathway is involved, while it provides a new target for treatment of periodontitis
induced by IL-17. |
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