| 吴琪 张静 曹留霞 刘晓萍 陈琛 卞晶晶.EGCG对LPS刺激人肺腺癌A549细胞凋亡及CUGBP1蛋白表达的影响[J].现代生物医学进展英文版,2014,14(18):3433-3437. |
| EGCG对LPS刺激人肺腺癌A549细胞凋亡及CUGBP1蛋白表达的影响 |
| Effect of EGCG on the Apoptosis and CUGBP1 Protein Expression ofHuman Lung Adenocarcinoma A549 Cells induced by LPS |
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| DOI： |
| 中文关键词: LPS 人肺腺癌细胞株A549 增殖 CUGBP1 表没食子儿茶素-3- 没食子酸酯(EGCG) 细胞凋亡 |
| 英文关键词: LPS Lung adenocarcinoma cell A549 Proliferation CUGBP1 Epigallocatechin-3-galate(EGCG) Cell apoptosis |
| 基金项目:山东省自然科学基金项目(ZR2012CM008) |
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| 中文摘要: |
| 目的：研究表没食子儿茶素-3-没食子酸酯(epigallocatechin-3 -gallate，EGCG)对炎性刺激的人肺腺癌A549 细胞增殖和凋亡
的影响及与CUGBP1 表达的关系。方法：MTT 法检测EGCG和LPS 刺激A549 细胞增殖活性的影响；流式细胞仪检测细胞凋亡；
免疫细胞化学检测EGCG 对LPS刺激人肺腺癌A549 细胞内CUGBP1 蛋白的表达。结果：与对照组相比，LPS体外显著促进A549
细胞增殖，其胞核胞质内CUGBP1 表达明显增强(P<0.01)。加入EGCG 可拮抗LPS 促A549 细胞增殖的作用，促进其凋亡，明显抑
制LPS刺激的A549细胞内CUGBP1的表达(P<0.01)。CUGBP1蛋白定量分析可知EGCG 和LPS 共同孵育A549细胞4h、24h
时，细胞中的CUGBP1 蛋白表达量较单纯LPS 作用时降低。但EGCG 和LPS 共同孵育A549 细胞24h，A549 细胞中胞核
CUGBP1 蛋白表达量(1210.565± 3.46)较4h 时胞核CUGBP1 蛋白表达量（67.344± 3.68)高，差异有统计学意义（t=927.164,
P<0.001)。结论：EGCG可能通过干扰CUGBP1 基因的表达抑制炎症刺激人肺腺癌细胞A549 的增殖，促进其凋亡。 |
| 英文摘要: |
| Objective:To study the effects of epigallocatechin-3-gallate (EGCG) on the apoptosis and CUGBP1 expression of
human lung adenocarcinoma A549 cells stimulated by lipopolysaccharide (LPS).Methods:The proliferation and apoptosis of human
lung adenocarcinoma A549 cells stimulated by LPS and EGCG were detected by MTT assay and flow cytometry respectively. The
CUGBP1 protein expression of A549 cells stimulated by LPS and EGCG were examined by immunocytochemistry (ICC).Results:LPS
markedly stimulated the proliferation of A549 cells and increased CUGBP1 protein expression in the nucleus and cytoplasm, compared
with normal control (P<0.01); EGCG inhibited the proliferation and obviously induced the apoptosis of A549 cells treated with LPS;
EGCG also markedly antagonized the CUGBP1 protein expression in nucleus of A549 cells incubated with LPS (P<0.01); Quantitative
analysis for the CUGBP1 protein level showed that at 4h or 24h, EGCG significantly inhibited the CUGBP1 expression of A549 cells
stimulated by LPS. The relative expression amount of CUGBP1 protein stimulated by EGCG and LPS at 24 h (1210.565± 3.46) was
significantly higher than that at 4h(67.344± 3.68) (t=927.164, P<0.001).Conclusion:EGCG can reverse the promotional effects of LPS
on the A549 cell proliferation and CUGBP1 expression and induce the apoptosis. |
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