| 谢貌 石书婧 王仁君 唐军 朱美婵 唐安洲.苦参碱对鼻咽癌细胞凋亡及凋亡相关基因p53 的表达研究[J].现代生物医学进展英文版,2014,14(18):3414-3417. |
| 苦参碱对鼻咽癌细胞凋亡及凋亡相关基因p53 的表达研究 |
| Effects of Matrine on the Apoptosis and the Apoptosis- Related-Gene p53Expression in Human Nasopharyngeal Carcinoma Cell Line in Vitro |
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| DOI： |
| 中文关键词: 苦参碱 鼻咽癌细胞 细胞凋亡 p53 |
| 英文关键词: Martine NPC cell Apoptosis p53 |
| 基金项目:国家自然科学基金项目（81241113）；教育部高等学校博士学科点专项科研基金资助项目（20094503110003） |
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| 中文摘要: |
| 目的：探讨苦参碱对体外培养的人鼻咽癌细胞增殖、凋亡及凋亡相关基因p53 mRNA 和蛋白表达的影响，初步探讨苦参碱
诱导人鼻咽癌细胞凋亡的可能机制。方法：采用MTT 法检测不同浓度苦参碱（0、0.25、0.5、1、1.5、2 mg/ml）对CNE1、CNE2 细胞增
殖的影响；采用荧光定量PCR 法检测这些浓度的苦参碱处理48 h后CNE2 细胞p53 mRNA的变化; Western Blot 检测其蛋白的
变化情况。结果：MTT结果显示苦参碱具有抑制CNE1、CNE2 细胞体外增殖作用，其抑制率存在浓度、时间依赖性。荧光定量
PCR及Western Blot 检测结果显示，苦参碱抑制CNE2细胞p53 mRNA 和蛋白的表达，且亦呈浓度依赖性。结论：苦参碱抑制
CNE2 细胞的增殖，诱导细胞凋亡，呈现浓度、时间依赖性，其作用与抑制CNE2 细胞中p53 基因和蛋白的表达密切相关。 |
| 英文摘要: |
| Objective:This study aims to evaluate the effects of matrine on human nasopharyngeal carcinoma cell line
proliferation，apoptosis and apoptosis-related-gene p53 expression and explore the mechanismof conceivable apoptosis promoted
by matrine.Methods: In vitro and apoptosis-related-gene p53 expression and explore the mechanismof conceivable apoptosis promoted
by matrine. o experiments, MTT assay was used to detect the effect of matrine on CNE2 cell proliferation. The
expression levels of p53 gene were detected by real-time PCR and western blot analysis.Results:MTT results showed that matrine
inhibited CNE2 cells proliferation in a dose and time-dependent fashion. The expression of p53 mRNA was significantly decreased in a
dose-dependent fashion (P<0.01). The expression of p53 protein was inhibited in a dose-dependent fashion (P<0.01).Conclusion:Matrine inhibits CNE2 cells proliferation and induces apoptosis in a dose- and time-dependent fashion, and the decreasing expression of
p53 mRNA and protein may closely relate to the mechanism. |
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