| 裴彤刘占涛郭锡春刘坤高华△.低分子硫酸软骨素在兔软骨修复过程中对IL-1β、TNF-α和TGF-β及钙磷的影响
和TGF-茁及钙磷的影响*[J].现代生物医学进展英文版,2014,14(17):3255-3258. |
| 低分子硫酸软骨素在兔软骨修复过程中对IL-1β、TNF-α和TGF-β及钙磷的影响
和TGF-茁及钙磷的影响* |
| Influence of Low Molecular Weight Chondroitin Sulfate on IL-1β, TNF-αand TGF-β, Calciumand Phosphate in the Process of CartilageReconstruction of Rabbit* |
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| DOI： |
| 中文关键词: 低分子量硫酸软骨素 软骨修复 IL-1β TNF-α 磷 |
| 英文关键词: Low molecular weight of chondroitin sulfate Cartilage reconstruction IL-1β TNF-α Phosphate |
| 基金项目:山东省自然基金项目(Y2008C16) |
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| 中文摘要: |
| 摘要目的：探讨低分子量硫酸软骨素(CS)在兔软骨修复过程中对于IL-1β、TNF-α和TGF-β及血液中钙磷含量的影响。方法：36
只成年新西兰大白兔，随机分成6 组，分别为对照组、模型组、低分子量CS低剂量组、低分子量CS 高剂量组、高分子量CS低剂
量组、高分子量CS高剂量组，每组6 只。通过在实验兔股骨髁关节面部位，钻出直径3mm，深度3mm的缺孔，造成其关节软骨的
缺损。术后次日给予药物进行灌胃，每日1次，5 周后取材。采用酶联免疫法测定关节液中IL-1β、TNF-α和TGF-β的含量，同时用
全自动生化分析仪及其配套的试剂盒来测定兔血清中钙，磷含量。结果：低分子量CS 和高分子量CS都能够减少关节液中炎性因
子的含量和增加TGF-β的含量，且与模型组相比具有统计学意义(P<0.05)；同时低分子量CS 与高分子量CS相比效果较好
(P<0.05)；而高低剂量之间无统计学意义(P>0.05)。与模型组相比，给予CS的组别，其血清中的钙磷含量相对较少(P<0.05)，但都高
于对照组。结论：高、低分子量的CS都可以增加TGF-β的含量，降低IL-1β、TNF-α和血清中钙、磷的含量。而对于软骨修复，这可
能是通过对上述因子的影响，从而产生积极的作用。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the influence of low molecular weight chondroitin sulfate on IL-1β, TNF-αand TGF-,
calcium and phosphate in the process of cartilage reconstruction of rabbit.Methods: 36 New Zealand rabbits were randomly divided into
6 groups with 6 rabbits in each group: control group, model group, low-dose of low molecular weight CS group, high-dose of low
molecular weight CS group, low-dose of high molecular weight CS group and high-dose of high molecular weight CS group. A defect,
3mm diameter, 3mm depth, was drilled at the condyles of femur of the hind legs by using an electric bone drill. After the next day for
animal drug perfusion, 1 time a day. Drawn after 5 weeks. The expression of cytokines, such as IL-1β, TNF-αand TGF-βin synovial
fluid was detected by ELISA, determining levels of calcium and phosphorus in serum of rabbit by automatic biochemical analyzer kit.
Results:Low molecular weight and high molecular weight CS were able to reduce levels of inflammatory cytokines and increase levels of
TGF-βin the joint fluid. And compared with that in the model group, there is statistical significance (P<0.05). While low molecular
weight CS' effect were better than high molecular weight CS (P<0.05). However, there was no obvious difference between high and low
dose (P>0.05). Compared with that in the model group, the groups of CS, which levels of calcium and phosphorus in the serum were
relatively low (P<0.05), but their levels were higher than that in the control group. However, these indexes had no obvious difference
between low molecular weight CS group and high molecular weight CS. Conclusion:High and low molecular weight CS can increase the
levels of TGF-βand reduce the levels of IL-1β, TNF-αand calcium, phosphate in the serum. For cartilage reconstruction, the above
factors may have positive effect. |
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