| 张广芬徐世霞孙合亮张明强王星明周志强△.谷氨酸及γ- 氨基丁酸在氯胺酮致大鼠精神分裂样表现中
的变化*[J].现代生物医学进展英文版,2014,14(17):3220-3222. |
| 谷氨酸及γ- 氨基丁酸在氯胺酮致大鼠精神分裂样表现中
的变化* |
| Changes of Glutamate and GABA in Ketamine-inducedPsychosis-like Responses in Rats* |
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| DOI： |
| 中文关键词: 氯胺酮 精神分裂症 前额皮层 谷氨酸 γ- 氨基丁酸 |
| 英文关键词: Ketamine Schizophrenia Prefrontal cortex Glutamate Gamma-aminobutyric acid |
| 基金项目:国家自然科学基金项目（30872424）；全军“十二五”科研面上项目（CWS11J017） |
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| 中文摘要: |
| 摘要目的：观察大鼠前额皮层谷氨酸（Glu）及-γ氨基丁酸（GABA）在氯胺酮致精神分裂样表现中的变化。方法：雄性Wistar 大鼠
32 只随机分为生理盐水组（S组，n=16）和氯胺酮组（K 组，n=16）。腹腔注射生理盐水或氯胺酮30 mg/kg（容积1 mL），一天一次，
连续5 天，最后一次给药后0.5 h及2 h分别通过敞箱实验观察大鼠自主活动，并记录刻板行为评分。行为学测试后，取大鼠前额
皮层，检测Glu 及GABA含量。结果：与S 组比较，K 组大鼠给药后0.5 h 自主活动增强、刻板行为评分增高（P<0.05），符合精神分
裂症大鼠表现；给药后2 h行为学评分则无显著差异（P>0.05）；给药后0.5 h及2 h 大鼠前额皮层Glu 水平均增加、GABA水平均
下降（P<0.05）。与氯胺酮给药后0.5 h组相比，给药后2 h Glu 水平下降（P<0.05）、GABA水平则无显著差异（P>0.05）。结论：氯胺
酮致精神分裂样表现可能与大鼠前额皮层Glu 增加及GABA 减少有关。 |
| 英文摘要: |
| ABSTRACT Objective:To observe the changes of prefrontal glutamate and gamma-aminobutyric acid (GABA) levels in ketamineinduced
psychosis-like responses in rats. Methods:Thirty two male Wistar rats were randomized into saline group (S group, n=16) and
ketamine group (K group, n=16). Saline 1 ml and ketamine 30 mg/kg (1 ml) were intraperitoneally given every day for 5 days.
Autonomic activities were observed during open field test at 0.5 h and 2 h after the last administration, the scores of stereotyped behavior
were also recorded. The prefrontal cortex was harvested after behavioral test for the determination of glutamate and GABA levels.
Results:Compared with the S group, rats in the K group showed significant increase of autonomic activities at 0.5 h after the last administration,
scores of stereotyped behavior showed significant increase (P<0.05), which were consistent with the psychosis performance in rats.
Behavioral scores had no significant difference at 2 h after the last administration between the two groups (P>0.05). The prefrontal
glutamate level increased and GABA level decreased at 0.5 h and 2 h after ketamine administration (P<0.05). Compared with the K
group of 0.5 h after administration, the K group of 2 h had a lower level of glutamate (P<0.05), but had no significant difference in
respect of GABA level (P>0.05).Conclusion: Increased glutamate level and decreased GABA level in rats prefrontal cortex may
contribute to ketamine-induced psychosis-like responses in rats. |
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