| 侯国伟 刘晓菲 张哲男 赵志威 王宁 姜涛 朴大勋.脾加骨髓细胞移植治疗小鼠大肠癌的实验研究[J].现代生物医学进展英文版,2014,14(16):3005-3009. |
| 脾加骨髓细胞移植治疗小鼠大肠癌的实验研究 |
| Experiment Research on the Spleen and Bone Marrow Cell Transplantationin the treatment of Colorectal Cancer in Mice |
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| DOI： |
| 中文关键词: 骨髓细胞移植 化疗 大肠癌 移植物抗宿主病 移植物抗肿瘤效应 |
| 英文关键词: Bone marrow transplantation Chemotherapy Colorectal cancer Graft-versus-host disease(GVHD) Graft-versus- tumor
effect(GVT) |
| 基金项目:基金项目：国家自然科学基金项目（30972878） |
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| 中文摘要: |
| 目的：探讨半相合脾加骨髓细胞移植治疗小鼠大肠癌的效果及其对嵌合体水平和移植物抗宿主病（GVHD）的影响。方法：以
接种CT26大肠癌细胞的BALB/c× C57BL/6杂交Fl 代雌性小鼠为受鼠，以健康雌性Fl、雄性C57BL/6、雄性C3H 小鼠为MHC
全相合、半相合、不相合供鼠，观察移植后的抑瘤情况；另设5 只化疗联合半相合脾加骨髓细胞移植的小鼠为监测组，用来作嵌合
体的分析，观察各组GVHD的情况。结果：经化疗预处理的脾加骨髓细胞移植组小鼠肿瘤明显缩小，与单纯化疗未进行移植组比
较，差异具有统计学意义(P＜0.05)；化疗联合半相合脾加骨髓细胞移植的小鼠于移植3 周后达完全供者植入，于移植后第10 天左
右出现纳差、倦怠、步态不稳、脱毛、腹泻、体重明显下降等GVHD的症状。结论：化疗预处理联合脾加骨髓细胞移植能对CT26 大
肠癌细胞产生GVT 效应，并伴随着GVHD及嵌合率的变化。 |
| 英文摘要: |
| Objective: To investigate the anti-tumor effects and the effect condition of the chimera level and GVHD of
transplanting MHC half-matched bone marrow cells combined with spleen cells in a murine model bearing colorectal cancer.Methods:Female (BALB/exC57BI/6)FI mice (CB6FI, H-2Kb/d) were subcutaneously inoculated with CT26 cells to develop a solid tumor model.
The syngenic, half-matched, and mismached donor bone marrow cells were from healthy female FI, maleC57BL/6 (H-2Kb), and male
C3H (H-2K)mice, respectively. The tumor weights were measured after transplantation. Meanwhile, the testing-group which received
chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells was used to analyze the chimera level.
And GVHD were observed after the transplantation.Results:The tumor weights of mice undergone chemotherapy pretreatment, bone
marrow cells combined with spleen cells were significantly decreased compared with those of the mice receiving chemotherapy alone
(P＜0.05). The mice which received chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells
attained donor implanted completely in 3 weeks after transplantation. Then began to fall ill at 10 days post-transplantation, GVHD
symptoms including decreased appetite, tired,instability of gait, unhairing, lose weight, diarrhea.Conclusion:The tumor weights of mice undergone chemotherapy pretreatment, bone
marrow cells combined with spleen cells were significantly decreased compared with those of the mice receiving chemotherapy alone
(P＜0.05). The mice which received chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells
attained donor implanted completely in 3 weeks after transplantation. Then began to fall ill at 10 days post-transplantation, GVHD
symptoms including decreased appetite, tired,instability of gait, unhairing, lose weight, diarrhea. |
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