| 赵保1 吴峰2 郑林丰3 叶晶亮1 韩瑞章1 苏国军1△.胶质瘤患者血浆miR-21的表达水平及意义*[J].现代生物医学进展英文版,2014,14(15):2879-2881. |
| 胶质瘤患者血浆miR-21的表达水平及意义* |
| Expression and Significance of Plasma miR-21 in Patients with Glioma* |
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| DOI： |
| 中文关键词: 胶质瘤 miR-21 血浆 诊断 标志物 |
| 英文关键词: Glioma miR-21 Expression Clinical stage |
| 基金项目:上海交通大学医工交叉项目（YG2011MS47） |
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| 中文摘要: |
| 摘要目的：研究脑胶质瘤患者血浆中miR-21 的水平，并评价其作为临床诊断标志物的可能性。方法：收集临床确诊的胶质瘤患
者和健康对照人群的血浆标本，常规方法提取血浆中RNA，采用茎环结构的引物将其逆转录成cDNA，实时定量PCR 的方法分
别检测miR-21 在胶质瘤患者和健康对照人群中的水平，根据ROC分析评价miR-21 作为诊断标志物的可能性。结果：分别收集
了胶质瘤患者和健康对照人群血浆标本52 例和43 例，实时定量PCR 结果显示，相对于健康对照人群，miR-21 在胶质瘤患者血
浆中呈高水平状态。ROC 分析说明血浆miR-21 水平作为诊断胶质瘤标志物具有较高的灵敏度和特异度。结论：血浆miR-21 水
平具有作为胶质瘤诊断的生物标志物的潜能。 |
| 英文摘要: |
| ABSTRACT Objective:To detect levels of plasma miR-21 and evaluate its possibility for clinical diagnostic biomarkers in patients
with glioma. Methods:The plasma samples were collected frompatients with glioma and healthy controls, and RNA was isolated by general
methods. Then the cDNA of miR-21 was generated by reverse transcription with stem-loop primer, and real-time PCR was applied to
detect the levels of plasma miR-21 in patients with glioma and healthy controls.Results: Of 52 plasma samples from glioma patients and
43 samples from healthy controls, the plasma miR-21 showed higher levels in glioma patients compared with the plasma from healthy
controls (P<0.05). Furthermore, ROC analysis certified that plasma miR-21 showed high sensitivity and specificity.Conclusion: Plasma
levels of miR-21 maybe served as a potential biomarker for glioms diagnosis. |
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