| 王元刚1 汤海峰2 张赟3 李娟1 李博1 高振辉1 王啸洋2 程光1△ 费舟1.太白银莲花皂苷B 对胶质瘤细胞生长抑制的研究*[J].现代生物医学进展英文版,2014,14(15):2812-2815. |
| 太白银莲花皂苷B 对胶质瘤细胞生长抑制的研究* |
| Saponin B, a Cytostatic Compound Purified fromAnemone Taipaiensis，Inhibit Proliferation Obviously in Human Glioblastoma Cell Line* |
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| DOI： |
| 中文关键词: 太白银莲花皂苷B 胶质细胞瘤 增殖抑制 |
| 英文关键词: Anemone taipaiensis Human glioblastoma Inhibiting proliferation |
| 基金项目:国家自然科学基金项目（30873402, 81274029）；陕西省自然科学基金项目（2012JM4010） |
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| 中文摘要: |
| 摘要目的：太白银莲花皂苷B（Anemone taipaiensis saponin B）是第一次从太白银莲花中经过系统化学分析和分离鉴定的皂苷之
一，所以它的生物学效应目前仍然不清楚。在本研究中，我们首次体外研究太白银莲花皂苷B对胶质瘤细胞系的生物学效应，观
察它对胶质瘤细胞增殖的的抑制作用。方法：采用四甲基偶氮唑蓝(MTT)法测定太白银莲花皂苷B 对胶质瘤细胞生长曲线的影
响，Hoechst 33342 细胞核染色后荧光显微镜观察，采用光学显微镜观察细胞的形态学变化。结果：MTT 实验结果显示太白银莲花
皂苷B 对胶质瘤细胞U87MG 和U251MG 有强烈的生长抑制作用，且具有剂量依赖性，应用SPSS18.0 统计软件得出太白银莲花
皂苷B 对U87MG 细胞72 h 的抑制浓度为IC25= 5.2 μmol/L, IC50 = 6.7 μmol/L and IC75= 8.7 μmol/L，U251 细胞的抑制浓度为
IC25= 6.2 μmol/L, IC50= 7.9 μmol/L and IC75= 10.5 μmol/L。Hoechst 33342 细胞核染色荧光显微镜观察以及光学显微镜下细胞形态
观察显示出典型的凋亡细胞形态学特征，经过皂苷B 处理后，细胞皱缩成圆球形，细胞核碎裂或者致密浓染，向核膜边缘聚集，染
色质浓缩为半月状、车轮状或者马蹄状，凋亡小体出现。这些特征在24 h时更明显。结论：体外实验初步显示, 太白银莲花皂苷B
对U87MG 和U251MG细胞具有明显的增殖抑制作用,并具有促凋亡作用。 |
| 英文摘要: |
| ABSTRACT Objective:Anemone taipaiensis saponin B was first isolated from Anemone taipaiensis, a ferine plant of the Qinling
mountains of southern Shaanxi province, China, that is distributed in the hill country of the fertile slopes or rocky grasslands at altitudes
between 2900 and 3700 m. in this paper, we first investigated the impact of saponin B to the human glioblastoma cell, as an in vitro
model to explore the effects of saponin B on GBMcell growth and apoptosis. Methods:the 3- (4, 5-dimethyl)-2, 5 diphenyl tetrazolium
bromide (MTT) assay was used to evaluate the cell proliferation, apoptosis was analyzed after staining of the cells with Hoechst 33342
(Sigma Aldrich) and follow fuorescence microscopy. Results:The results of MTT showed that saponin B significantly suppressed
U87MG and U251MG cell Proliferation. For U87MG cells, the inhibitory concentrations of saponin B at 72 h were found to be IC25= 5.2
μmol/L, IC50 = 6.7 μmol/L and IC75= 8.7 μmol/L. For U251MG cells, the inhibitory concentrations were IC25 = 6.2 μmol/L, IC50 = 7.9
μmol/L and IC75= 10.5 μmol/L(SPSS 18.0). Chromatin condensation and the apoptotic bodies observed by fiuorescence microscopy, We
determined the residual cell viability and number of apoptotic cells in glioma U87MG cells after treatment with saponin B at the
concentrations of IC25, IC50, and IC75 for 8 and 24 h. Typical of the induction of apoptosis were observed: (a) weak and irregularly shaped
marginal chromatin condensation; (b) highly condensed nuclear chromatin that was inverted in one side; (c) relatively compact and
irregularly shaped marginal chromatin condensation. under light microscopy, with increasing concentrations of saponin B, cells were
increasingly found in rounded form.Conclusion: As pointed out by the experimental results, saponin B is an efficient cytostatic agent of
glioblastoma cells, may be considered a novel compound can inhibit glioma cell growth and survival. |
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